Ab157304

We collected 1.0×10⁶ KASUMI-1 cells, washed them with PBS and resuspended them. Then, we added 20 μL mouse anti-human monoclonal antibodies CD34-PE (ab157304, Abcam, dilution: 1: 4000) and CD38-FITC (ab1173, Abcam, dilution: 1: 6000) and the sample was incubated at room temperature in the dark for 30 min, and then washed with PBS. A flow cytometer (Becton Dickerson, San Jose, CA, USA) was used to test and sort CD34⁺/CD38⁻ cells, and FITC and PE fluorescent antibodies were used as isotype controls. The same method was applied to detect the sorted cells.

Human MSCs single-cell suspensions were harvested using a 0.05% trypsin/
Ethylenediaminetetraacetic acid (EDTA) solution; after FBS neutralization incubated in
blocking buffer [1% FBS in Dulbecco’s phosphatebuffered saline (DPBS)] for 30 minutes.
Next, 1×10⁶ cells were separately incubated for 1 hour at 4°C with an optimal
dilution of conjugated antibodies that included anti-CD73-FITC (ab28061), anti-CD45- FITC
(ab27287), anti-CD90-FITC (ab11155), antiCD34-PE (ab157304), and anti-CD105-PE (ab91138),
all from Abcam (Cambridge, UK). Flow cytometry experiments were performed with a BD
FACSCalibur Flow Cytometer (BD Biosciences) and data analyzed by the Flowing software.