To visualize the extent of aggregation and morphology of the different combinations, samples were diluted to 10μM in PBS and quickly adsorbed on to glow-discharge carbon-coated grids. Transmission electron microscopy was performed in a Jeol 120-kV JEM-1400 microscope, using 1% uranyl acetate for negative staining.
120 kv jem 1400 microscope
Manufactured by JEOL
The JEOL 120-kV JEM-1400 microscope is a transmission electron microscope (TEM) that operates at an accelerating voltage of 120 kilovolts. It is designed to provide high-resolution imaging and analysis of a wide range of materials.
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2 protocols using 120 kv jem 1400 microscope
1
Visualizing Aggregation and Morphology
2
Visualizing scFv-h3D6 aggregate morphology
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To visualize the aggregation extent and morphology of the scFv-h3D6-Ec and scFv-h3D6-Pp aggregates, incubation of 100-μM samples was carried out at 37°C for 48h. Then, samples were 1:10 diluted in PBS and quickly adsorbed onto glow-discharge carbon-coated grids. TEM was performed in a Jeol 120-kV JEM-1400 microscope, using 1% uranyl acetate for negative staining.
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