Lipopolysaccharide (from E. coli, L2654), nigericin, BzATP, arachidonic acid (10931), cis-5,8,11,14,17-eicosapentaenoic acid (EPA, E2011), linoleic acid (LA, L1376), oleic acid (OA, O1008), mecamylamine hydrochloride, and strychnine hydrochloride were obtained from Sigma-Aldrich (Taufkirchen, Germany), thapsigargin, ATK, and BEL from Enzo Life Sciences (Lausen, Switzerland), α-bungarotoxin from Tocris Bioscience (Bristol, UK), and Prolastin® from Grifols (Frankfurt, Germany). Conotoxins RgIA4 and ArIB were described previously (11 (link)–13 (link)). Polyclonal goat-anti-AAT was purchased from Bethyl (A80-122A, Montgomery, AL, USA), monoclonal rabbit anti-CD36 antibodies (clone D8L9T) from Cell Signaling Technologies (Danvers, MA, USA), and mouse anti-β-actin antibodies (clone A2228) from Sigma-Aldrich. Horseradish peroxidase-labeled secondary antibodies, rabbit anti-mouse Ig, rabbit anti-goat Ig, and goat anti-rabbit Ig, were provided by Dako (Glostrup, Denmark). The C-terminal peptide of AAT (C-36, corresponding to residues 359–394) was synthesized by JPT Peptide Technologies GmbH (Berlin, Germany) and provided with a purity of >95% (HPLC).
Goat anti rabbit ig
Manufactured by Agilent Technologies
Sourced in Denmark
Goat anti-rabbit Ig is a secondary antibody used in various immunological techniques to detect the presence of rabbit primary antibodies. It is a polyclonal antibody that binds to the immunoglobulin (Ig) of rabbits, allowing for the amplification and visualization of rabbit-specific signals.
Automatically generated - may contain errors
Lab products found in correlation
Nigericin, by Merck Group (2 mentions)
Mecamylamine hydrochloride, by Merck Group (2 mentions)
Strychnine hydrochloride, by Merck Group (2 mentions)
Thapsigargin, by Enzo Life Sciences (2 mentions)
α bungarotoxin, by Bio-Techne (2 mentions)
Bzatp, by Merck Group (2 mentions)
Rabbit anti mouse ig, by Agilent Technologies (2 mentions)
Prolastin, by Grifols (2 mentions)
Anti ezrin, by Cell Signaling Technology (1 mentions)
Ready set go, by Thermo Fisher Scientific (1 mentions)
Hrp conjugated rabbit anti mouse ig, by Agilent Technologies (1 mentions)
Enhanced chemi luminescence (ecl), by Cytiva (1 mentions)
Chemidoc mp imaging system, by Bio-Rad (1 mentions)
Aquatex, by Boehringer Ingelheim (1 mentions)
Bz 9000, by Keyence (1 mentions)
4 protocols using goat anti rabbit ig
1
Characterization of Protease Inhibitor Interactions
2
Western Blot and ELISA Analyses
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Western blot analyses were performed as previously described [27 (link),62 (link)]. Following incubation with primary antibody, the membranes were washed, and the proteins visualized following 60 min incubation at room temperature with HRP-conjugated rabbit anti-mouse Ig, swine anti-rabbit Ig or rabbit anti-goat Ig (P0260, P0399 and P0449 DAKO, Glostrup, Denmark) using ECL (Amersham Biosciences) technology. The primary antibodies used were anti-VDR, anti-CD3ζ, anti-CTLA-4 and anti-albumin (D-6, 6B10.2, C-19 and F-8, Santa Cruz Biotecnology), anti-PLC-γ1 (05–163, Upstate Biotechnology), anti-ezrin (3145, Cell Signaling Technology) and anti-DBP (SAB2501100, Sigma Aldrich). For band density quantification ECL exposed sheets were analysed in a ChemiDoc MP Imaging System from Bio-Rad. Measurement of the cytokines IL-13 and IFN-γ were determined by ELISA according to the manufacturer’s protocol (Ready-Set-Go; eBioscience).
3
Histone H1 and Octamer Binding Assay
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Maxisorp microtiter plates were coated with 20 μg/mL human histone H1 (Sigma) or full length histone core octamers (i.e., H2A/H2B/H3/H4, Sigma) in PBS overnight, at 4°C. As negative control, 20 μg/mL A1AT was immobilized. Between each of the following steps, the plates were washed four times with 0.1% BSA-PBST. After coating, plates were blocked in 3% BSA-PBST for 1 h at RT and incubated with distinct concentrations of C3, or C3b, or C3a, or BSA in 0.1% BSA-PBST for 2 h at RT. After incubation, bound proteins were detected using rat anti-human C3d (1,000X in Quench, C3 and C3b detection) or rabbit anti-human C3a (20,000X in Quench, C3a detection), followed by HRP-conjugated rabbit anti-mouse Ig (2,000X in Quench) or goat anti-rabbit Ig (10,000X in Quench, DAKO). As substrate, OPD was used and absorbance at 490 nm was measured as described above.
4
MUC8 and TNF Expression in Salivary Glands
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For immunohistochemistry, reactivity was followed with an antibody against the mucin peptide core epitope of MUC8 and an antibody against TNF in tissue sections (7 μm) from the parotid and submandibular glands. The antibody targeting MUC8 was studied on sections subjected to 10 min of microwave heating pretreatment as described previously [17 (link)]. Primary antibodies against MUC8 (Proteintech, Manchester, UK, 55489-1-AP, 1:250, polyclonal) and TNF (Santa Cruz Biotechnology, Inc., Dallas, TX, USA, 52B83, 1:50, monoclonal) were used. All primary antibodies were applied overnight at room temperature (RT). Secondary antibodies goat anti-rabbit IG (1:200, Dako, Glostrup, Denmark) or goat anti-mouse (1:200; Dako, Glostrup, Denmark) were incubated for at least 4 h at RT. Visualization was performed with peroxidase-labeled streptavidin-biotin for at least 5 min. After counterstaining with hemalum, sections were mounted in Aquatex (Boehringer, Germany). Two negative control sections were used in each case; one was incubated with secondary antibody only, the other with primary antibody only. Nasal epithelium and thymus, respectively, were used as positive controls. All slides were examined with a microscope (Keyence, BZ9000, Osaka, Japan).
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