Egfp rab11

Manufactured by Addgene

EGFP-RAB11 is a fluorescently-labeled variant of the small GTPase Rab11. Rab11 is a key regulator of endocytic recycling pathways. The EGFP-tagged Rab11 can be used to visualize the dynamics and localization of Rab11 in live cells.

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Lab products found in correlation

Apex2, by Addgene (1 mentions)

Close spelling variants of this product

RAB11

1 protocol using egfp rab11

FGFR2b and Rab11 Localization Constructs

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

eGFP-RAB11 (Addgene #12674); eGFP-RAB11_S52N (mutagenesis of eGFP-RAB11^{13 (link)}); eGFP (Addgene #34680); dynamin-2_K44A-eGFP (mutagenesis of Dynamin-eGFP^{13 (link)}); HA-FGFR2b^{13 (link)}; APEX2 (Addgene #49386); pCDH-EF1-HA-FGFR2b-T2A-mApple and pCDH-EF1-HA-FGFR2b-APEX2-T2A-mApple were generated for this study using the pCDH-mApple backbone^{88 (link)}. Inserts were PCR amplified, digested and subcloned into the multiple cloning site using MfeI/EcoRI and NotI adaptors. For HA-FGFR2b-APEX2, APEX2 (SalI adaptors) was amplified, digested and subcloned into pDisplay HA-FGFR2b^{13 (link)}. eGFP-RAB11-APEX2 and eGFP-APEX2 were PCR amplified, digested and subcloned into pcDNA4 (gift form Jennet Gummadova) using EcoRI and XbaI acceptors.

Watson J., Ferguson H.R., Brady R.M., Ferguson J., Fullwood P., Mo H., Bexley K.H., Knight D., Howell G., Schwartz J.M., Smith M.P, & Francavilla C. (2022). Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival. Nature Communications, 13, 6589.

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