Amounts of sCD46 in culture supernatants was measured by sandwich ELISA (detection range: 7.8–500 ng/mL): anti-CD46 (clone GB-24),38 and biotin anti-CD46 (clone MEM-258, Novus Biologicals). IgM and IgG in B cell tissue culture supernatants were analyzed using the human IgM (detection range: 15.6–1000 ng/mL) and IgG (detection range 7.8–1000 ng/mL) ELISA quantification (Bethyl Laboratories). MMP-9 in T cell tissue culture supernatants was measured using the LegendMax™ ELISA (BioLegend; detection range: 62.5–4000 pg/mL). C3b was analyzed using the Complement C3b human ELISA (Abcam; detection range: 0.15–10 ng/mL).
Complement c3b human elisa
Manufactured by Abcam
The Complement C3b human ELISA is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure the levels of complement component C3b in human samples. The assay utilizes specific antibodies to capture and detect the target analyte.
Automatically generated - may contain errors
2 protocols using complement c3b human elisa
1
Quantification of Immune Mediators
2
Quantitative Immunoassays for Biomarkers
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Amounts of sCD46 in culture supernatants was measured by sandwich ELISA (detection range: 7.8–500 ng/mL): anti‐CD46 (clone GB‐24),38 and biotin anti‐CD46 (clone MEM‐258, Novus Biologicals). IgM and IgG in B cell tissue culture supernatants were analyzed using the human IgM (detection range: 15.6–1000 ng/mL) and IgG (detection range 7.8–1000 ng/mL) ELISA quantification (Bethyl Laboratories). MMP‐9 in T cell tissue culture supernatants was measured using the LegendMaxTM ELISA (BioLegend; detection range: 62.5–4000 pg/mL). C3b was analyzed using the Complement C3b human ELISA (Abcam; detection range: 0.15–10 ng/mL).
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