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11 protocols using mncl2 4h2o

1

Synthesis of Metal-Organic Compounds

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Citric acid (CA), polyvinyl pyrrolidone (PVP K-30), acetone, Cu(NO3)2, MnCl2·4H2O, CaCl2, ZnCl2, FeSO4·7H2O, Fe(NO3)3·9H2O, NaCl, MgCl2, Ni(NO3)2·6H2O, AgNO3, Pb(NO3)2, KNO3, Co(NO3)2·6H2O, Cd(NO3)2·4H2O, and Hg(NO3)2·H2O were all purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Quinine sulfate was purchased from Aladdin Chemistry Co., Ltd. All the chemicals were of analytical grade and were used as received without any further purification. High-purity deionized water with a resistivity of greater than 18 MΩ cm−1 was obtained from the Millipore system and was used throughout the experiments.
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2

Synthesis and Characterization of Zr-Based Hybrid Materials

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2-aminoterephthalic acid (ATA, 98%), tetracycline (TET, ≥98%), doxycycline (DOX, ≥98%), oxytetracycline (OTC, ≥98%), chlortetracycline (CTC, ≥98%), chloramphenicol (CAP, 98%), tyrosine (Tyr), histidine (His), serine (Ser), phenylalanine (Phe), arginine (Arg) and cysteine (Cys) were provided by Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Zirconium chloride (ZrCl4, 99%) was purchased from Beijing Yinuokai Technology Co., Ltd. (Beijing, China). Methacrylic acid (MAA, 99%), 2,2-azobisisobutyronitrile (AIBN, 99%) and ethylene glycol dimethacrylate (EGDMA, 98%) were obtained from TCI Development Co., Ltd. (Shanghai, China). N, N-dimethylformamide (DMF), ethanol, glucose, sucrose, MnCl2·4H2O, MgCl2·6H2O, Zn(NO3)2·6H2O, CaCl2, NaCl and KCl were supplied by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All reagents and chemicals used in this work were of at least analytical grade. The apparatuses utilized in this study are described in detail in the supporting information.
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3

Synthesis of Metal-Organic Frameworks

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Melamine, Zn(NO3)2·6H2O, HgCl2, CaCl2, NaCl, FeCl2, FeCl3, KCl, MnCl2·4H2O, Li(CH3COO)2, SnCl2, CoCl2·6H2O, AgNO3, CuCl2, Ni(NO3)2·6H2O were ordered from Sinopharm Chemical Reagent Co., Ltd. Tetrahydrofuran (THF), 2-methylimidazole (Hmim), dimethylformamide (DMF), ethanol, methanol, and acetone were purchased from Sigma Aldrich. All the other chemicals were analytical pure grade or better quality. Deionized water (18.2 MΩ cm−1) produced by a Milli-Q system (Bedford, MA) was used throughout.
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4

Silkworm Excrement: A Versatile Bioresource

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Silkworm excrement was obtained from sericulturists (Zhejiang, China) and washed to remove impurities. AgNO3, AlCl3·6H2O, CaCl2, CdCl2·2.5H2O, CrCl3·6H2O, FeCl2·4H2O, FeCl3·6H2O, KCl, LiCl, MnCl2·4H2O, NaCl, NH4Cl, NiCl2·6H2O, PbCl2, ZnCl2 were provided by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Glucose, α-Lactose, sucrose, dopamine hydrochloride, β-alanine, l-arginine, l-asparagine, l-glutamic acid, glycine, l-histidine, l-methionine, l-phenylalanine, l-proline, dl-homocysteine, l-cysteine, reduced l-glutathione of analytical reagent grade were purchased from Aladin Ltd. (Shanghai, China). The fetal calf serum (main compositions: globular protein and bovine serum albumin) was obtained from Tianhang Biotechnology Co., Ltd. (Zhejiang, China). Ultrapure water (18.2 MΩ cm−1) obtained from the Millipore system was used in this study.
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5

Synthesis of Sulfur-based Nanomaterials

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Sublimed sulfur, polyethylene glycol (PEG-400), Tetramethylammonium hydroxide (TMA·OH), and NaOH were provided by Shanghai Aladdin Biochemical Technology Co. (Shanghai, China). MnCl2·4H2O, K2S2O8 were acquired from Sinopharm Chemical Reagent Co., Ltd. (Tianjin, China). N2H4 (v/v 80%) was purchased from Sigma Chemical Co., Ltd. (Shanghai, China). The prepared solutions of all experiments used ultrapure water (18.2 MΩ cm) from a water purification system.
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6

Garlic-based Heavy Metal Removal

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Fresh garlic were purchased from local supermarket (Changchun, Jilin Province). Ethylenediamine (EA) was attained from Aladdin. Na2S·9H2O were purchased from Xi Long Chemical Reagent Co., Ltd. Na2SO4, NaCl, KCl, AlCl3, ZnCl2, Ba(NO3)2, FeCl3, MgCl2, Ni(NO3)2·6H2O, MnCl2·4H2O, CuCl2·2H2O,PbCl2, and HgCl2 were obtained from Sinopharm Chemical Reagent Co., Ltd. and used without further purification.
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7

Cyanobacteria Bioremediation with Metal Ions

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Cyanobacteria were collected from Taihu, Wuxi, China. FeCl3 (99%). MgCl2·6H2O (98%) was purchased from Adamas-beta Co., Ltd. (Shanghai, China) and Shanghai Aladdin Reagent Co., Ltd. (Shanghai, China) K2CrO7 (99.8%), FeCl2·4H2O (99%), CuSO4·5H2O (99%), MnCl2·4H2O (98%), ZnSO4·7H2O (99.5%), and Hg(NO3)2·H2O (97.5%) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China) Deionized water, purified using a Millipore water purification system, was used in all the experiments. The HAs used in this study are pH 6.57, containing –COOH, –OH, –NH, and –C–O functional groups [28 (link)].
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8

Synthesis and Characterization of Metal-Polymer Complexes

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Carboxymethylcellulose (CMC, M. W. 90 000, 250 000, 700 000 g mol−1), 1,2-propanediamine and 1,6-hexamethylenediamine were procured from Aladdin Chemistry Co., Ltd. Ethylenediamine (EDA, 99% purity) was purchased from Kermel Analytical Reagent Co., Ltd. CrCl3·6H2O, MgCl2, AgNO3, KCl, MnCl2·4H2O, NaCl, ZnCl2, BaCl2·2H2O, AlCl3, FeCl3·6H2O, CuCl2·2H2O, CaCl2, FeCl2·4H2O were obtained from Sinopharm Chemical Reagent Co., Ltd. K2CrO4, K2Cr2O7, KMnO4, TiCl3, glutathione (GSH), ascorbic acid, SnCl4 and hydroxylamine hydrochloride were supplied by Macklin Biochemical Co., Ltd.
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9

Synthesis and Characterization of Antimicrobial Composites

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Zirconium chloride (ZrCl4, 98%) was obtained from Beijing Yinuokai Technology Co., Ltd (Beijing, China). N,N-Dimethylformamide (DMF), ethyl alcohol (EtOH), Zn(NO3)2·6H2O, AlCl3·6H2O, MgCl2·6H2O, MnCl2·4H2O, KCl, CaCl2, and NaCl were from SinopharmChemical Reagent Co., Ltd (Shanghai, China). 2-Aminoterephthalic acid (ATA, 98%), tetracycline (TET), doxycycline (DOX), oxytetracycline (OTC), ampicillin (AMP), amoxicillin (AMX), chloramphenicol (CM), cysteine (Cys), tyrosine (Tyr), phenylalanine (Phe), serine (Ser), arginine (Arg), and histidine (His) were purchased from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China), and the chemical structural formulas of TCs were displayed in Fig. S1. All chemical reagents used were of analytical grade and did not require further purification. During the entire study, doubly deionized water (DDW, 18.2 MΩ cm−1) obtained by a Milli-Q water purification system was used. The fresh milk sample applied in the experiment was purchased from a local supermarket.
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10

Heavy Metal Exposure Mitigation Protocol

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K2Cr2O7, MnCl2•4H2O, NiCl2•6H2O, CuSO4•5H2O, ZnSO4•7H2O, CdCl2•2.5H2O, CH3ClHg, CH3COO)2Pb•3H2O, TCD (>99%), 0.9% sterile normal saline, 40% formaldehyde solution were purchased from Sinopharm Chemical Reagent Co., Ltd (Beijing, China). CH3ClHg (analytical grade, ≥99.0%) was purchased from Dr. Ehrenstorfer GmbH (Augsburg, Germany). EGCG (90%) was purchased from Hangzhou Yibeijia Tea Co., Ltd (Hangzhou, China). GSH (98%) was purchased from Solarbio Life Sciences Co., Ltd. (Beijing, China). Nitric acid (HNO3, 68%, trace metal, UP grade) was purchased from Suzhou Crystal Clear Chemical Co., Ltd. (Suzhou, China). Male Sprague Dawley (SD) rats weighing 150–180 g were purchased from the Experimental Animal Center of Zhejiang Province. Inductively coupled plasma mass spectrometer (ICP-MS) (NexION™ 300D) was produced by PerkinElmer (USA).
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