To demonstrate the localization of proteins, cells were grown on Lab-Tek four-well glass chamber slides (catalog No. C6807; NUNC, USA). After 24 h incubation, cells were fixed and permeabilized with cold methanol for 5 min, washed with phosphate-buffered saline (PBS), and incubated with primary antibody (1:100) and secondary antibody conjugates (1:500). Images were collected on a laser scanning confocal microscope LSM710 (Carl Zeiss, Germany) equipped with argon (488 nm) and krypton (568 nm) lasers, using a 40 × water immersion objective lens. Images were processed with ZEN 2009 light edition (Carl Zeiss).
Lab tek four well glass chamber slides
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Lab-Tek four-well glass chamber slides are laboratory equipment designed for cell culture and microscopy applications. They provide a multi-well format for containing and observing cell samples under a microscope.
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Lab products found in correlation
2 protocols using lab tek four well glass chamber slides
1
Protein Localization in Cultured Cells
2
Immunofluorescence Imaging of HeLa Cells
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HeLa cells were grown on Lab-Tek four-well glass chamber slides (NUNC, Rockford, IL, USA) and were incubated in HBSS for the indicated time periods. Cells were fixed and permeabilized with cold methanol for 5 min. They were then washed with phosphate-buffered saline, and incubated with primary Ab and secondary Ab conjugates. Images were collected on a laser scanning confocal microscope LSM710 (Carl Zeiss, Oberkochen, Germany) equipped with argon (488 nm) and krypton (568 nm) lasers, using a × 40 water immersion objective lens. Images were processed with ZEN 2009 light edition (Carl Zeiss).
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