For detection of MHCA in Western blots, equal amounts of cell lysates were separated by SDS-PAGE, transferred to Immobilon-P-membrane (Serva), incubated in primary antibody in the presence of 5% skim milk in TBS-T (TBS with Tween 20) overnight, and labeled with HRP-coupled secondary antibodies.
Immobilon p membrane
Manufactured by Serva Electrophoresis
Sourced in Germany
Immobilon-P-membrane is a polyvinylodifluoride (PVDF) membrane used for protein transfer and immobilization in Western blotting and other protein detection techniques. It provides a stable and high-binding capacity surface for protein samples.
Automatically generated - may contain errors
Lab products found in correlation
Gelatin, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Serva Electrophoresis (1 mentions)
Triton x 100, by Serva Electrophoresis (1 mentions)
Thiourea, by Cytiva (1 mentions)
Urea, by Cytiva (1 mentions)
Tween 20, by Serva Electrophoresis (1 mentions)
Ipg buffer, by Cytiva (1 mentions)
3 3 cholamidopropyl dimethylammonio 1 propanesulphonate chaps, by Merck Group (1 mentions)
Dapi h 1200, by Vector Laboratories (1 mentions)
2 protocols using immobilon p membrane
1
Western Blot Detection of MHCA
2
Analytical-Grade Reagents for Proteomics
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Analytical-grade chemicals were utilized. BSA (albumin bovine fraction V, pH 7.0), Immobilon-P membrane, Tween 20, Triton X-100 and Coomassie Brilliant Blue (CBB) R-250 were obtained from Serva (Germany). Gelatin, dithiothreitol (DTT), iodoacetamide, 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulphonate (CHAPS) were from Sigma (Prague, Czech Republic); thiourea, urea and IPG buffer (pH 3–10) were purchased from Amersham Biosciences (Uppsala, Sweden). Protein standards were from Bio-Rad (Hercules, CA, USA), chemiluminescent substrate (SuperSignal, Pierce) was obtained from Rockford (USA) and VectaShield mounting medium for fluorescence with DAPI H-1200 from Vector Laboratories, (Burlingame, CA, USA).
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