Antibodies against trpv1

All drugs and antibodies were purchased from Sigma-Aldrich (St Louis, MO, USA) unless otherwise stated. Capsaicin (Cat # BML-EI125, Enzo Life Sciences, Farmingdale, NY, USA) was dissolved in ethanol as a 200 mM stock solution. For stereotaxic injection into the cerebral cortex, the capsaicin stock solution was diluted with PBS to the suitable concentration, for example, 1 mM for 1 nmol/μl injection. For in vitro application, capsaicin was diluted with PBS and immediately added to the medium. Resiniferatoxin (RTX, Cat # 1137, Tocris Bioscience, MO, USA) was dissolved in ethanol as a 50 mM stock solution and diluted with PBS just before use. Fura-2 AM ester (Cat # 50033, Biotium, Hayward, CA, USA) was dissolved in dimethyl sulphoxide (DMSO) to produce a 1 mM stock solution and diluted as indicated below just before use.
Antibodies against TRPV1 (Cat # sc-12498), GluN1 (Cat # sc-1467) and GluN2B (Cat # sc-1469) were purchased from Santa Cruz Biotechnology (CA, USA). An antibody against the transferrin receptor was purchased from Invitrogen (Cat # 13-6800, Carlsbad, CA, USA).