Phage particles from high titter stock suspensions (1010 PFU/mL) were negatively stained with 2% uranyl acetate (Electron Microscopy Sciences, Hatfield, UK) and subjected to electron micrographs using a JEOL 1011 transmission electron microscope (JEDL USA Inc., Peabody, MA, USA) operating at 100 kV. The images were obtained with a Gatan CCD–Erlangshen ES100W.
Es100w
Manufactured by Ametek
Sourced in United States
The ES100W is a lab equipment product manufactured by Ametek. It is designed to perform essential functions required in various laboratory settings. The core function of the ES100W is to provide reliable and accurate measurements, though the specific intended use is not explicitly stated.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using es100w
1
Negative Staining of Phage Particles
2
Glyphosate Effects on S. vacuolatus Morphology
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The morphology of S. vacuolatus was evaluated in vivo from cultures grown in BBM liquid medium that contained 0, 4, 6 or 8 mg L -1 of glyphosate. Lugol staining was applied to highlight the cell starch granules. The observation and photomicrography were performed with a Leica DM 500 light microscope equipped with a Leica ICC50 digital camera.
The ultrastructure from control and treated cells was analyzed by transmission electron microscopy. The ultrathin sections were observed and photographed with a Zeiss EM 109 T electron microscope equipped with a Gatan ES100W digital camera at the Instituto de Biología Celular y Neurociencias (LANAIS -MIE), Facultad de Medicina, Universidad de Buenos Aires.
The number of nuclei and the cell wall thickness of at least 40 randomly chosen control and trated (8 mg L -1 ) cells were measured in the ultrathin sections photographed. The percentage of cells with more than one nucleus and the average cell wall thickness were calculated.
Additional details on procedure for ultrathin section preparation are presented in Supplementary material.
The ultrastructure from control and treated cells was analyzed by transmission electron microscopy. The ultrathin sections were observed and photographed with a Zeiss EM 109 T electron microscope equipped with a Gatan ES100W digital camera at the Instituto de Biología Celular y Neurociencias (LANAIS -MIE), Facultad de Medicina, Universidad de Buenos Aires.
The number of nuclei and the cell wall thickness of at least 40 randomly chosen control and trated (8 mg L -1 ) cells were measured in the ultrathin sections photographed. The percentage of cells with more than one nucleus and the average cell wall thickness were calculated.
Additional details on procedure for ultrathin section preparation are presented in Supplementary material.
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