Freestyle freedom lite glucometer

Animal studies were approved by the UCLA Office of Animal Research Oversight. The transgenic mouse strain expressing hIAPP (hTG), available from Jackson Laboratory, Bar Harbor, ME, United States: IMSR cat. No. JAX:008232, RRID:IMSR_JAX:008232) is described elsewhere (Janson et al., 1996 (link)). hTG males develop diabetes between 9 and 12 weeks of age (Gurlo et al., 2016 (link)). The wild-type FVB mice (WT; IMSR cat. No. CRL:207, RRID:IMSR_CRL:207) were originally purchased from Charles Rivers Laboratory (Wilmington, MA, United States). All mice were bred at UCLA, and were maintained on a 12-h day/night rhythm with Harlan Teklad Rodent Diet 8604 (Placentia, CA, United States) and water ad libitum. Heterozygous hIAPP transgenic mice (hTGhet) were obtained by cross-breeding of hTG male to FVB female. Diabetes was monitored by measurement of blood glucose in a tail-tip blood sample with a FreeStyle Freedom Lite Glucometer (Abbott, Alameda, CA, United States). The number of mice used for each experiment is provided in the figure legends.

Mice were fasted overnight for 16 h. 20% Pyruvate solution was prepared in Dulbecco´s phosphate buffered saline (PBS) (Gibco, USA). Mice were intraperitoneally injected with 2 g/kg pyruvate and blood glucose levels were measured at 0, 15, 30, 60, 90, 120 min time points using Freestyle Freedom lite glucometer (Abbott).

Blood glucose measurement and urinary albumin and creatinine assays were performed as described previously (18 (link)). Briefly, blood glucose was measured from tail vein blood by using a FreeStyle Freedom lite glucometer (Abbott, Abbott Park, IL). For urinary albumin and creatinine measurements, spot urine samples were collected non-invasively from mice. Urinary albumin and creatinine concentrations were measured using a mouse albumin ELISA (Bethyl Laboratories, Montgomery, TX) and a creatinine assay (Exocell, Philadelphia, PA), respectively. Subsequently, protein concentrations and urine albumin-to-creatinine ratios were calculated.