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Nissl cresyl violet acetate

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Nissl (cresyl violet acetate) is a histological stain used in the field of neuroscience. It is a cationic dye that specifically binds to the rough endoplasmic reticulum (Nissl substance) in the perikarya of neurons. This staining technique allows for the visualization and identification of neuronal cell bodies and their morphological characteristics.

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Lab products found in correlation

Typhoon phosphorimager, by Cytiva (2 mentions) Optimum cutting temperature compound, by Sakura Finetek (1 mentions) Oct compound, by Sakura Finetek (1 mentions) Tissue tek oct, by Sakura Finetek (1 mentions) Palm microbeam laser microdissection system, by Zeiss (1 mentions) Cm3050 s, by Leica (1 mentions)

Spelling variants of this product

Cresyl violet (348 citations) Cresyl violet acetate (120 citations)

3 protocols using nissl cresyl violet acetate

1

Mapping Brain and Spinal Cord Biodistribution

Cited 1 time
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Spinal cords and brain tissues from mice used for biodistribution were further analyzed through digital autoradiography. After perfusion and gamma counting, brain tissue was frozen in optimum cutting temperature compound (Sakura Finetek, Inc, Torrance, CA), and 20-µm-thick sections were cut using a cryostat microtome HM500 (Microm, Walldorf, Germany) at −20°C. Whole spinal cords and slides with brain slices were laid in a cassette and exposed to a 18F-sensitive storage phosphor film imaging plate (Fujifilm; GE Healthcare) for 20 hours at −20°C. Plates were scanned using a typhoon phosphorimager (Amersham Biosciences, Piscataway, NJ) and analyzed using ImageJ (image processing software, version 2.0.0). Brain section anatomy was confirmed by staining the exact same sections with Nissl (cresyl violet acetate; Sigma Aldrich, St. Louis, MO), as previously described.25 (link)
Cropper H.C., Johnson E.M., Haight E.S., Cordonnier S.A., Chaney A.M., Forman T.E., Biswal A., Stevens M.Y., James M.L, & Tawfik V.L. (2019). Longitudinal translocator protein-18 kDa–positron emission tomography imaging of peripheral and central myeloid cells in a mouse model of complex regional pain syndrome. Pain, 160(9), 2136-2148.
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2

Radiotracer Biodistribution in Mouse CNS

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Spinal cords and brain tissues from mice used for biodistribution were further analyzed via digital autoradiography. Following perfusion and gamma counting, brain tissue was frozen in Optimum Cutting Temperature (O.C.T.) compound (Sakura Finetek, Inc.) and 20 μm-thick sections were cut using a cryostat microtome HM500 (Microm, Germany) at −20°C. Whole spinal cords and slides with brain slices were laid in a cassette and exposed to a 18F-sensitive storage phosphor film imaging plate (Fujifilm, GE Healthcare) for 20 hours at −20°C. Plates were scanned using a typhoon phosphorimager (Amersham Biosciences, USA) and analyzed using ImageJ (image processing software, version 2.0.0). Brain section anatomy was confirmed by staining the exact same sections with Nissl (cresyl violet acetate, Sigma Aldrich), as previously described.25
Cropper H.C., Johnson E.M., Haight E.S., Cordonnier S.A., Chaney A.M., Forman T.E., Biswal A., Stevens M.Y., James M.L, & Tawfik V.L. (2019). Longitudinal translocator protein-18 kDa–positron emission tomography imaging of peripheral and central myeloid cells in a mouse model of complex regional pain syndrome. Pain, 160(9), 2136-2148.
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3

Lung Tissue Preparation for RT-PCR

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For real-time RT-PCR analysis, the lungs were removed en bloc, inflated with diluted Tissue-Tek OCT (Sakura Finetek USA., Torrance, CA, USA) (50% vol/vol) in ribonuclease (RNase)-free phosphate-buffered saline with 10% sucrose, immediately frozen in liquid nitrogen, and stored at -80°C [26 (link)]. The lungs were sectioned (12 μm) with a cryostat (Leica CM3050 S, Leica Biosystems, Wetzlar, DEU) and stained with 0.5% Nissl (Cresyl violet acetate) (Sigma Chemical, St. Louis, MO, USA)/0.1 M sodium acetate buffer for laser capture microdissection (LCM) of the bronchiolar epithelial cells using a Zeiss PALM Microbeam Laser Microdissection System (Carl Zeiss Microscopy GmbH, Jena, DEU). A total of 20,000 to 40,000 bursts of the laser were used to collect a minimum of 5,000 cells from each animal.
Ito J.T., Cervilha D.A., Lourenço J.D., Gonçalves N.G., Volpini R.A., Caldini E.G., Landman G., Lin C.J., Velosa A.P., Teodoro W.P., Tibério I.D., Mauad T., Martins M.D., Macchione M, & Lopes F.D. (2019). Th17/Treg imbalance in COPD progression: A temporal analysis using a CS-induced model. PLoS ONE, 14(1), e0209351.
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