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Pe cy7 anti mouse cd31 clone 390

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PE-Cy7 anti-mouse CD31 (clone 390) is a fluorochrome-conjugated monoclonal antibody that binds to the mouse CD31 antigen. CD31, also known as platelet endothelial cell adhesion molecule (PECAM-1), is a cell surface glycoprotein expressed on endothelial cells, platelets, and some leukocytes.

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Lab products found in correlation

Pe cy7 anti mouse cd45 clone 30 f11, by BD (2 mentions) Pe anti mouse cd106 vcam 1, by Thermo Fisher Scientific (2 mentions) Facsaria 2, by BD (2 mentions) Collagenase type 2, by Thermo Fisher Scientific (1 mentions) Dispase, by Thermo Fisher Scientific (1 mentions) Collagenase type 2, by Worthington (1 mentions)

Close spelling variants of this product

PE-Cy7 rat anti-mouse CD31 (clone 390), PE-Cy7 anti-mouse CD31 CD31 (clone 390, Anti-CD31 PE-Cy7 CD31-PE-Cy7 Anti-CD31-PE Anti-mouse CD31 CD31-PE Anti-CD31 Mouse anti

2 protocols using pe cy7 anti mouse cd31 clone 390

1

Isolation and Characterization of Satellite Cells

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Satellite cells were isolated from hindlimb and forelimb muscles as previously described (Eliazer et al., 2019 (link)). The mononuclear muscle cells were stained for PE-Cy7 anti-mouse CD31 (clone 390; BD Biosciences), PE-Cy7 anti-mouse CD45 (clone 30-F11; BD Biosciences), APC-Cy7 anti-mouse Sca1 (clone D7; BD Biosciences), PE anti-mouse CD106/VCAM-1 (Invitrogen), and APC anti-α7 integrin (clone R2F2; AbLab). Fluorescence-assisted cell sorting (FACS) was performed using FACS Aria II (BD Biosciences) by gating for CD31−/CD45−/Sca1−/α7 integrin+/VCAM1+ to isolate SCs. SCs from Pax7-nGFP mouse were sorted for GFP fluorescence. The GFP+ gate was divided into top 15% (GFPhigh fraction), middle 45% (Pax7medium fraction), and bottom 15% (Pax7low fraction). The isolated SCs were fixed immediately at t0 or cultured in growth media (Ham’s F10 media, 20% fetal bovine serum, 5 ng/ml FGF2) containing 10 μm EdU for 60 hr (cell cycle entry assay) or cultured in plating media (DMEM with 10% horse serum) for 3 days (differentiation assay).
Eliazer S., Sun X., Barruet E, & Brack A.S. (2022). Heterogeneous levels of delta-like 4 within a multinucleated niche cell maintains muscle stem cell diversity. eLife, 11, e68180.
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2

Isolation of Satellite Cells from Skeletal Muscles

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Satellite cells were isolated from hindlimb and forelimb muscles. The mice were euthanized, the hindlimb and forelimb skeletal muscles were removed, chopped finely and digested using 0.2% Collagenase type 2 (Worthington) in DMEM media for 90 minutes in shaking water bath at 37°C. The digested muscle was washed twice with Rinsing media (Ham’s F10, 10% horse serum). A second digestion was performed with 0.2% Collagenase type 2 and 0.4% Dispase (Gibco) in Rinsing media for 30 minutes in shaking water bath at 37°C. The digested tissue was passed through a 20-gauge needle three times, then passed through a 40μm filter and a 20μm filter. The mononuclear muscle cells were stained for PE-Cy7 anti-mouse CD31 (clone 390; BD Biosciences), PE-Cy7 anti-mouse CD45 (clone 30-F11; BD Biosciences), APC-Cy7 anti-mouse Seal (clone D7; BD Biosciences), PE anti-mouse CD106/VCAM-1 (Invitrogen) and APC anti-α7 integrin (clone R2F2; AbLab). FACS was performed using FACS Aria II (BD Biosciences) by gating for CD31−/CD45−/Scal−/α7 integrin+/VCAM1+ to isolate SCs.
Eliazer S., Muncie J.M., Christensen J., Sun X., D’Urso R.S., Weaver V.M, & Brack A.S. (2019). Wnt4 from the niche controls the mechano-properties and quiescent state of muscle stem cells. Cell stem cell, 25(5), 654-665.e4.
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