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Fsc a

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The FSC-A is a lab equipment product that measures the forward scattered light of a sample. It is used to analyze particle size and distribution within a sample.

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Lab products found in correlation

Streptavidin allophycocyanin, by BD (2 mentions) Ficoll isopaque lymphoprep, by Axis-Shield (2 mentions) Fitc conjugated anti cd45ro uchl1, by BD (2 mentions) Pe conjugated anti cd25 m a251, by BD (2 mentions) Percp cy5.5 conjugated anti cd4 sk3, by BD (2 mentions) Biotinylated anti foxp3 236a e7, by Thermo Fisher Scientific (2 mentions) Ssc a, by BD (2 mentions) Fl1 a, by BD (2 mentions) Fl2 a, by BD (2 mentions) Fl3 a, by BD (2 mentions) Fl4 a, by BD (2 mentions) Lsrfortessa, by BD (2 mentions)

2 protocols using fsc a

1

Phenotypic Analysis of T-Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMC were isolated with Ficoll-Isopaque (Lymphoprep™; Axis-Shield, Oslo, Norway) gradient centrifugation. All cells were freshly stained with the following monoclonal antibodies and analysed promptly as previously described (9 (link)): FITC-conjugated anti-CD45RO (UCHL1; BD Biosciences); PE-conjugated anti-CD25 (M-A251; BD Biosciences); PerCP-Cy5.5-conjugated anti-CD4 (SK3; BD Biosciences); and biotinylated anti-FOXP3 (236A/E7; eBioscience, San Diego, CA) and allophycocyanin–streptavidin (BD Biosciences). The second dataset (Table I) was obtained by LSR Fortessa (BD Biosciences), using the following settings for voltage: FSC-A (319), SSC-A (335), FL1-A (CD45RO, 582), FL2-A (CD25, 478), FL3-A (CD4, 742), and FL4-A (FOXP3, 676). FlowJo (Tree Star) was used for manual gating.
Fujii H., Josse J., Tanioka M., Miyachi Y., Husson F, & Ono M. (2016). Regulatory T Cells in Melanoma Revisited by a Computational Clustering of FOXP3+ T Cell Subpopulations. The Journal of Immunology Author Choice, 196(6), 2885-2892.
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2

Isolation and Phenotyping of Human PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs were isolated with Ficoll–Isopaque (Lymphoprep; Axis-Shield, Oslo, Norway) gradient centrifugation. All cells were freshly stained with the following mAbs and analyzed promptly as previously described (9 (link)): FITC-conjugated anti-CD45RO (UCHL1; BD Biosciences); PE-conjugated anti-CD25 (M-A251; BD Biosciences); PerCP-Cy5.5–conjugated anti-CD4 (SK3; BD Biosciences); and biotinylated anti-FOXP3 (236A/E7; eBioscience, San Diego, CA) and allophycocyanin-streptavidin (BD Biosciences). The second data set (Table I) was obtained by LSR Fortessa (BD Biosciences), using the following settings for voltage: FSC-A (319), SSC-A (335), FL1-A (CD45RO, 582), FL2-A (CD25, 478), FL3-A (CD4, 742), and FL4-A (FOXP3, 676). FlowJo (Tree Star) was used for manual gating.
Fujii H., Josse J., Tanioka M., Miyachi Y., Husson F, & Ono M. (2016). Regulatory T Cells in Melanoma Revisited by a Computational Clustering of FOXP3+ T Cell Subpopulations. The Journal of Immunology Author Choice, 196(6), 2885-2892.
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