Mcc950 pz0280

Aβ42 was purchased form the Peptide Institute (Ibaraki, Osaka, Japan). Anti-Aβ mouse monoclonal antibody (6E10) and anti-mouse immunoglobulin G (IgG) were purchased from BioLegend (San Diego, CA, USA) and R&D Systems (Minneapolis, MN, USA), respectively. Thioflavin T (ThT) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Anti-FLAG monoclonal antibody M2 (F1804) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Human IL-1β ELISA Set II was purchased from BD Biosciences (San Jose, CA, USA). Anti-cleaved caspase-1 rabbit monoclonal antibody (Asp297) (D57A2) (S4199) was purchased from Cell Signaling Technology (Danvers, MA, USA). MCC950 (PZ0280) was purchased from Sigma-Aldrich (St. Louis, MO). Isoliquiritigenin (I0822) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).

Fetal bovine serum (FBS), Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute (RPMI)-1640 culture medium and penicillin–streptomycin were purchased from GE Healthcare Life Sciences (Marlborough, MA, USA). IL-1β ELISA kit (Human 88-7261-22) and TRIzol reagent (15596018) was purchased from Invitrogen (Carlsbad, CA, USA). Lipopolysaccharide (LPS; L6529), palmitate (P9767) and MCC950 (PZ0280) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Pioglitazone was purchased from Takeda Pharmaceutical (AD-4833, Kanagawa, Japan). Ezetimibe was obtained from Cayman Chemical (16331, Ann Arbor, MI, USA). The triglyceride assay kit was purchased from Bioassay systems (ETGA-200, Hayward, CA, USA). Anti-F4/80 antibody was obtained from Abcam (ab6640, Cambridge, MA, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Georgiachem (MT 1036, Norcross, GA, USA).