For time-lapse microscopy experiments, cells were seeded into 96 well glass bottom plates with 200 μL of media two days prior to imaging (Brooks Automation Inc. 96 Well Glass Bottom Black Plates (MGB09612LGL)). A 10 μL volume of stress perturbing molecules diluted in media was added to wells at various time points before imaging. MG132 was used at final concentrations ranging from 0.06 μM to 10 μM to induce proteasome inhibition. STA9090 (Ganetespib) was used at concentrations ranging from 6.25 nM to 500 nM to induce HSP90 inhibition. Time-lapse images were taken with the GE IN Cell Analyzer 6000, Perkin-Elmer Operetta High-Content Imaging System, or Nikon Eclipse Ti Live Cell Imaging System. Cells were kept in a controlled chamber of 37°C temperature and 5% CO2. Time intervals between images varied based on experiment, ranging from 5 to 30 minutes (exact intervals are noted in the Figure legends).
Eclipse ti live cell imaging system
Manufactured by Nikon
The Eclipse Ti Live Cell Imaging System is a microscope designed for live cell imaging. It provides high-resolution imaging capabilities for observing cellular processes in real-time.
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Lab products found in correlation
2 protocols using eclipse ti live cell imaging system
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Time-lapse microscopy of stress response
2
Time-lapse microscopy of stress response
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For time-lapse microscopy experiments, cells were seeded into 96 well glass bottom plates with 200 μL of media two days prior to imaging (Brooks Automation Inc. 96 Well Glass Bottom Black Plates (MGB09612LGL)). A 10 μL volume of stress perturbing molecules diluted in media was added to wells at various time points before imaging. MG132 was used at final concentrations ranging from 0.06 μM to 10 μM to induce proteasome inhibition. STA9090 (Ganetespib) was used at concentrations ranging from 6.25 nM to 500 nM to induce HSP90 inhibition. Time-lapse images were taken with the GE IN Cell Analyzer 6000, Perkin-Elmer Operetta High-Content Imaging System, or Nikon Eclipse Ti Live Cell Imaging System. Cells were kept in a controlled chamber of 37°C temperature and 5% CO2. Time intervals between images varied based on experiment, ranging from 5 to 30 minutes (exact intervals are noted in the Figure legends).
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