Western blot was performed as previously reported (27 (link)). The following antibodies were used for immunoblotting: OX40L (14991S), p105/p50 (13681S), p65 (6956S), RelB (10544S), c-Rel (67489S), HDAC1 (5356S), HSP90 (4877S), P-STAT3 (Tyr705, 9145S), STAT3 (12640S), P-STAT6 (Tyr641, 56554S), STAT6 (5397S), β-Actin-HRP (5125S), Goat anti-Rabbit IgG-HRP (7074S), Horse anti-Mouse IgG-HRP (7076S) from Cell Signaling Technology; p100/p52 (05–361) from Millipore; StarBright Blue 700 Goat anti-Mouse IgG (12004159), StarBright Blue 520 Goat anti-Rabbit IgG (12005870) from Bio-Rad; VeriBlot-HRP (ab131366) from Abcam. Images were acquired and analyzed using Bio-Rad ChemiDoc MP Imaging System.
Goat anti rabbit igg hrp 7074s
Manufactured by Cell Signaling Technology
Goat anti-Rabbit IgG-HRP (7074S) is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and quantify rabbit primary antibodies in various immunoassays.
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Lab products found in correlation
P stat6, by Cell Signaling Technology (1 mentions)
β actin hrp, by Cell Signaling Technology (1 mentions)
Veriblot hrp, by Abcam (1 mentions)
Horse anti mouse igg hrp 7076s, by Cell Signaling Technology (1 mentions)
P stat3, by Cell Signaling Technology (1 mentions)
Chemidoc mp imaging system, by Bio-Rad (1 mentions)
Goat anti rabbit igg, by Bio-Rad (1 mentions)
Mouse monoclonal anti tubulin antibody clone 236 10501, by Thermo Fisher Scientific (1 mentions)
Donkey anti goat hrp conjugated antibody ft 1i7890, by Interchim (1 mentions)
Alexa fluor 488 conjugated anti rabbit igg, by Abcam (1 mentions)
Rabbit monoclonal anti pd l1 clone sp142, by Roche (1 mentions)
2 protocols using goat anti rabbit igg hrp 7074s
1
Western Blot Analysis of NF-κB Signaling
2
Comprehensive Antibody Panel for Protein Detection
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The rabbit polyclonal anti‐SPARC (15274‐1‐AP) and the mouse monoclonal anti‐periostin (clone No 1A11A3) antibodies were purchased from Proteintech. The mouse monoclonal anti‐SPARC (clone AON‐5031, sc‐73 472) and the mouse monoclonal anti‐HSC70 (clone B‐6, sc‐7298) antibodies were purchased from Santa Cruz Biotechnology. The mouse monoclonal anti‐tubulin antibody (clone 236‐10 501, #A11126) was from Thermo Fisher Scientific. The mouse monoclonal anti‐cytokeratin 5/6 antibody (clone 6D5/16 B4) was from Dako. The mouse monoclonal anti‐epidermal growth factor receptor (EGFR) antibody (clone 31G7) was from inVitroGen. The mouse monoclonal anti‐PD‐1 (clone MRQ‐22), and the mouse monoclonal anti‐CD163 (clone 10D6) antibodies were from BioSB. The rabbit monoclonal anti‐PD‐L1 (clone SP142) was from Roche. The horse anti‐mouse immunoglobulin G (IgG)‐horseradish peroxidase (#7076), and goat anti‐rabbit IgG‐HRP (#7074 S) secondary antibodies were from Cell Signaling Technology. The donkey anti‐goat HRP conjugated antibody (FT‐1I7890) was from Interchim. The Alexa Fluor 488‐conjugated anti‐rabbit IgG (#Ab150077) was purchased from Abcam, and the Alexa Fluor 594‐conjugated anti‐mouse IgG (711‐585‐152) from ImmunoResearch Laboratories. Hoechst 33342 (#FP‐BB1340) was from Interchim FluoProbes.
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