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Rna extraction reagent kits dp441

Manufactured by Tiangen Biotech
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Sourced in China

The RNA extraction reagent kits DP441 are designed for the efficient extraction and purification of total RNA from a variety of biological samples. The kits utilize a guanidinium thiocyanate-phenol-chloroform extraction method to isolate high-quality RNA that is suitable for downstream applications such as RT-PCR, Northern blotting, and microarray analysis.

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Lab products found in correlation

Primescript rt master mix, by Takara Bio (2 mentions) Pmd19 t vector, by Takara Bio (1 mentions)

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RNA extraction reagent kit DP441 Kit RNA extraction kit RNA reagent kit DP441 RNA kits

2 protocols using rna extraction reagent kits dp441

1

Cloning of C. nitidissima Genes

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RNA extraction was performed using RNA extraction reagent kits DP441 (TIANGEN, Beijing, China), and the cDNA library of C. nitidissima was built using a PrimeScript RT Master MIX (Takara, Dalian, China). According to transcriptome sequencing data, primer premier 5 was used for primer design, and the full-length coding sequences (CDS) of genes were amplified from the cDNA library and cloned into pMD19-T vectors (Takara, Dalian, China). The recombinant vector was transferred into DH5α competent cells of Escherichia coli (Takara, Dalian, China), and positive clones were confirmed by sequencing. Primers for gene cloning are listed in Supplementary Table S1.
Yu S., Li J., Peng T., Ni S., Feng Y., Wang Q., Wang M., Chu X., Fan Z., Li X., Yin H., Ge W, & Liu W. (2022). Identification of Chalcone Isomerase Family Genes and Roles of CnCHI4 in Flavonoid Metabolism in Camellia nitidissima. Biomolecules, 13(1), 41.
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2

Comparative qRT-PCR Analysis of C. nitidissima and N. tabacum

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The total RNA from the leaves of C. nitidissima and N. tabacum was extracted using RNA extraction reagent kits DP441 (TIANGEN, Beijing, China). The total RNA was reverse transcribed to cDNA using a PrimeScript RT Master Mix (Takara, Dalian, China). qRT-PCR analyses were conducted as previously described [35 (link)], and the 2−ΔΔCT method was used for quantitative analysis. The sequences of the primers used for qRT-PCR are depicted in Supplementary Table S4.
Yu S., Li J., Peng T., Ni S., Feng Y., Wang Q., Wang M., Chu X., Fan Z., Li X., Yin H., Ge W, & Liu W. (2022). Identification of Chalcone Isomerase Family Genes and Roles of CnCHI4 in Flavonoid Metabolism in Camellia nitidissima. Biomolecules, 13(1), 41.
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