A standard Th1/Th2/Th17 assay (BD Biosciences, The Netherlands) was used to determine cytokine concentrations in lung homogenates and supernatants of lung restimulation according to the manufacturer’s instructions. IL-33, CCL17, CCL20 and CCL22 were measured with a DuoSet ELISA (R&D Systems), and IL-13 and IL-5 with a Ready-SET-Go!® ELISA (eBioscience, USA). The concentrations of these cytokines were expressed as pg/mg protein in lung homogenates and pg/mL in BALF and restimulation supernatants.
Th1 th2 th17 assay
Manufactured by BD
The Th1/Th2/Th17 assay is a laboratory tool used to measure the levels of specific T-helper cell subsets, including Th1, Th2, and Th17 cells, in a given sample. The assay provides quantitative information about the relative proportions of these T-cell subtypes, which play important roles in the immune response.
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Lab products found in correlation
2 protocols using th1 th2 th17 assay
1
Th1/Th2/Th17 Cytokine Profiling in Lung Samples
2
Cytokine and Chemokine Profiling in Lungs
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IL-33, CCL20, CCL17, and CCL22 concentrations were measured in lung homogenates with a DuoSet ELISA (R&D Systems, USA) and IL-5 and IL-13 with a Ready-SET-Go!® ELISA (eBioscience, USA). A standard IL-13 flex set or a standard Th1/Th2/Th17 assay (BD Biosciences, The Netherlands) was used to determine cytokine concentrations in supernatants of lung restimulation. All assays were performed according to the manufacturer’s protocol. The concentrations of cytokines and chemokines were expressed as picogram per milligram protein in lung homogenates and picogram per milliliter in lung restimulation supernatants.
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