Cnt prime epithelial cell culture medium

Human primary foreskin keratinocytes were cultured in CnT-Prime Epithelial Cell Culture Medium (CELLnTEC, Bern, Switzerland). Human foreskin fibroblasts were cultured in Dulbecco’s Modified Eagle’s Medium DMEM (Sigma-Aldrich, St. Louis, MO), supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin.

The strain of T. vaginalis used in the present study was maintained in our laboratory and identified as actin genotype E by PCR restriction RFLP, which is the dominant genotype in the city of Xinxiang, Henan Province, China. As described in our previous studies [1 (link), 26 (link)], T. vaginalis was cultured in complete TYM medium in a humidified chamber maintained at 37 °C and 5% CO₂. The parasites were collected by centrifugation and used for subsequent research.
Human vaginal epithelial cells (cell line VK2/E6E7) were purchased from American Type Culture Collection (Manassas, VA, USA; ATCC #CRL-2616). The VK2/E6E7 cells were cultured on CnT-Prime Epithelial Cell Culture Medium (Cellntec, Bern, Switzerland) supplemented with 1% penicillin–streptomycin solution, in a humidified chamber at 37 °C and 5% CO₂. Cells of the 293T cell line were kept in our laboratory and cultured on Dulbecco's Modified Eagle Medium (DMEM) supplemented with 1% penicillin–streptomycin solution and 10% fetal calf serum in a humidified chamber at 37 °C and 5% CO₂.
Eight-week-old BALB/c mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) and kept under specific pathogen-free conditions.

Different human UCCs were used to represent the heterogeneity of UC: VM‐CUB1, RT‐112, UM‐UC‐3, J82, SW‐1710 and T24. UCCs were obtained from the DSMZ (Braunschweig, Germany) and Dr. H.B. Grossmann HB (Houston, USA). Two benign cell lines were also used as control: HBLAK, a spontaneously immortalized normal human uroepithelial cell line (Cellntec Advanced Cell Systems)
³⁶ (link) and VHF2 dermal fibroblasts.
³⁷ (link) UCC and VHF2 were cultivated in DMEM (Dulbecco's Modified Eagle Medium, 4.5 g/L D‐glucose, L‐glutamine, Gibco, Life Technologies Limited) supplemented with 10% heat inactivated fœtal bovine serum (Bio & Sell). HBLAK was cultivated in CnT Prime epithelial cell culture medium (Cellntec Advanced Cell Systems) and to detach the cells, accutase (Sigma‐Aldrich) was used.
Previously generated cisplatin‐resistant sublines J82 LTT, T24 LTT and RT‐112 LTT were maintained in cisplatin supplemented medium as recently described.
³⁸ (link) Phase contrast images of quisinostat or DMSO treated cells were taken with the Nikon Eclipse microscope (Nikon) and the NIS elements software.
The identity of the cells is regularly verified by STR (short tandem repeat) analysis, and cells were tested for mycoplasma contamination by PCR.