Human plgf quantikine elisa kit

Manufactured by R&D Systems

Sourced in United States

The Human PlGF Quantikine ELISA kit is a quantitative sandwich enzyme immunoassay designed for the measurement of human Placental Growth Factor (PlGF) concentrations in cell culture supernates, serum, and plasma.

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Lab products found in correlation

Human mouse rat activin a quantikine elisa kit, by R&D Systems (1 mentions) Human vegf quantikine elisa kit, by R&D Systems (1 mentions)

4 protocols using human plgf quantikine elisa kit

Quantifying PlGF Secretion by DSCs

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After 48-hour culture of freshly isolated DSCs, the concentration of PlGF was measured by Human PlGF Quantikine ELISA kit (R&D Systems) according to the manufacturer’s instructions.

Chang R., Dai J., Wang L., Liu H., Jiang H., Liu X., Jiang L., He F, & Hu L. (2023). PlGF/FLT-1 deficiency leads to reduced STAT3-C/EBPβ signaling and aberrant polarization in decidual macrophages during early spontaneous abortion. Frontiers in Immunology, 14, 1061949.

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Quantifying PIGF Levels in CSE-Treated Cells

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The cells were treated with CSE at the previously described doses for 2 h, followed by changing the media and a 4- to 24-h recovery. Cellular supernatants were analyzed using a Human PlGF Quantikine ELISA Kit (R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer’s protocols.

Wu D., Yuan Y., Lin Z., Lai T., Chen M., Li W., Lv Q., Yuan B., Li D, & Wu B. (2016). Cigarette smoke extract induces placental growth factor release from human bronchial epithelial cells via ROS/MAPK (ERK-1/2)/Egr-1 axis. International Journal of Chronic Obstructive Pulmonary Disease, 11, 3031-3042.

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Quantification of Angiogenic Factors

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The serum levels of Activin A, PlGF and sFLT1 were measured using commercial ELISA kits (R&D, Cat # DAC00B, Human/Mouse/Rat Activin A Quantikine ELISA Kit; Cat # DPG00, Human PLGF Quantikine ELISA Kit; Cat # DVR100C, Human VEGFR1/Flt-1 Quantikine ELISA Kit).

Lan X., Guo L., Zhu S., Cao Y., Niu Y., Han S., Li Z., Li Y, & Yan J. (2022). First-Trimester Serum Cytokine Profile in Pregnancies Conceived After Assisted Reproductive Technology (ART) With Subsequent Pregnancy-Induced Hypertension. Frontiers in Immunology, 13, 930582.

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Quantifying Serum Cytokines and Angiogenic Factors

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Serum IL-36β levels and supernatant levels of VEGF-A and PlGF were quantified using the human IL-36β/IL-1F8 DuoSet ELISA (R&D systems), human VEGF Quantikine ELISA Kit (R&D systems), and human PlGF Quantikine ELISA Kit (R&D systems) according to the manufacturer’s instructions. These assays employ the quantitative sandwich enzyme immunoassay technique.

Komaki R., Miyagaki T., Tanaka M., Nakajima K., Okano T., Takeuchi S, & Kadono T. (2023). Increased Interleukin-36β Expression Promotes Angiogenesis in Japanese Atopic Dermatitis. International Journal of Molecular Sciences, 24(13), 11104.

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