Hyclone dmem with high glucose

Cells were cultured in media as previously described [33 (link),34 (link)]. Briefly, human oral squamous cell carcinoma (OSCC) OC3 and TW2.6 cell lines were grown in Dulbecco’s Modified Eagle Medium (DMEM, Cytiva, Marlborough, MA, USA) containing Keratinocyte serum-free medium (KSFM, Gibco, Waltham, MA, USA) and Ham’s F-12 Nutrient Mix (ThermoFisher Scientific, Waltham, MA, USA), respectively, in a 1:2 ratio, and supplemented with 10% fetal bovine serum (FBS, Gibco, Waltham, MA, USA). Human OSCC OECM1 and human renal cell carcinoma 786-O were grown in RPMI 1640 (Cytiva, Marlborough, MA, USA) medium containing 10% FBS (Gibco, London, UK). Human renal cancer cell line Caki-1 (also known as HTB 46) was maintained in Hyclone DMEM with high glucose (Cytiva, Waltham, MA, USA) containing 10% FBS (Gibco, Waltham, MA, USA). All the media contain 100 units of penicillin, and 100 μg of streptomycin/mL. All cell lines were cultured at 37 °C in a humidified atmosphere of 5% CO2.

Cells were cultured in media as previously described [33 (link),34 (link)]. Briefly, human oral squamous cell carcinoma (OSCC) OC3 and TW2.6 cell lines were grown in Dulbecco’s Modified Eagle Medium (DMEM, Cytiva, Marlborough, MA, USA) containing Keratinocyte serum-free medium (KSFM, Gibco, Waltham, MA, USA) and Ham’s F-12 Nutrient Mix (ThermoFisher Scientific, Waltham, MA, USA), respectively, in a 1:2 ratio, and supplemented with 10% fetal bovine serum (FBS, Gibco, Waltham, MA, USA). Human OSCC OECM1 and human renal cell carcinoma 786-O were grown in RPMI 1640 (Cytiva, Marlborough, MA, USA) medium containing 10% FBS (Gibco, London, UK). Human renal cancer cell line Caki-1 (also known as HTB 46) was maintained in Hyclone DMEM with high glucose (Cytiva, Waltham, MA, USA) containing 10% FBS (Gibco, Waltham, MA, USA). All the media contain 100 units of penicillin, and 100 μg of streptomycin/mL. All cell lines were cultured at 37 °C in a humidified atmosphere of 5% CO2.