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Primer sets for il 33 and gapdh

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The primer sets for IL-33 and GAPDH are laboratory equipment designed for use in molecular biology and genomics research. The IL-33 primer set is used to amplify the IL-33 gene, which is involved in various immune and inflammatory processes. The GAPDH primer set is used to amplify the GAPDH gene, which is commonly used as a reference gene for gene expression analysis. Both primer sets are designed to provide reliable and consistent results in PCR and RT-PCR experiments.

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Lab products found in correlation

Superscript 2 reverse transcriptase, by Thermo Fisher Scientific (2 mentions) Rnalater, by Qiagen (2 mentions) Rneasy fibrous tissue mini kit, by Qiagen (2 mentions) Power sybr green master mix, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

GAPDH (170 citations) GAPDH primers (17 citations) IL-33 (6 citations) Primer sets (3 citations) Primers for GAPDH (2 citations)

2 protocols using primer sets for il 33 and gapdh

1

Quantification of IL-33 Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Normal skin or portions of wounds were placed into RNAlater (QIAGEN, Valencia, CA) and stored at −20°C prior to RNA isolation. Tissue was homogenized in RLT buffer and isolated using the RNeasy Fibrous Tissue mini kit (QIAGEN) according to manufacturer’s instructions. cDNA was generated using Superscript II Reverse Transcriptase (Invitrogen) according to manufacturer’s instructions. Subsequent real-time quantitative PCR was performed using Power SYBR Green Master Mix (Applied Biosystems, Grand Island, NY) and commercially available primer sets for IL-33 and GAPDH (QIAGEN). Relative quantification was achieved using the comparative CT method with GAPDH as the reference gene.
Rak G.D., Osborne L.C., Siracusa M.C., Kim B.S., Wang K., Bayat A., Artis D, & Volk S.W. (2016). IL-33-dependent group 2 innate lymphoid cells promote cutaneous wound healing. The Journal of investigative dermatology, 136(2), 487-496.
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2

Quantification of IL-33 Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Normal skin or portions of wounds were placed into RNAlater (QIAGEN, Valencia, CA) and stored at −20°C prior to RNA isolation. Tissue was homogenized in RLT buffer and isolated using the RNeasy Fibrous Tissue mini kit (QIAGEN) according to manufacturer’s instructions. cDNA was generated using Superscript II Reverse Transcriptase (Invitrogen) according to manufacturer’s instructions. Subsequent real-time quantitative PCR was performed using Power SYBR Green Master Mix (Applied Biosystems, Grand Island, NY) and commercially available primer sets for IL-33 and GAPDH (QIAGEN). Relative quantification was achieved using the comparative CT method with GAPDH as the reference gene.
Rak G.D., Osborne L.C., Siracusa M.C., Kim B.S., Wang K., Bayat A., Artis D, & Volk S.W. (2016). IL-33-dependent group 2 innate lymphoid cells promote cutaneous wound healing. The Journal of investigative dermatology, 136(2), 487-496.
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