Antibodies used in this study were mouse CD-MPR (1/100, 22d4, Developmental Studies Hybridoma Bank (DSHB)), mouse GalNAc-T2 (neat, UH-4, gift from U. Mandel and H. Clausen), rabbit GCC88 (1/300, HPA021323, Sigma), mouse CI-MPR (1/100, ab2733, Abcam), rabbit giantin (1/300, HPA011008, Sigma), mouse GM130 (1/300, 610823, BD Biosciences), mouse golgin-245 (1/200, 611281, BD Biosciences), rabbit golgin-84 (1/300, HPA000992, Sigma), rat HA (1/300, 3 F10, Roche), sheep TGN46 (1/300, AHP500, AbD serotec), rabbit TMF (1/300 HPA008729, Sigma), and rabbit ZFPL1 (1/500, HPA014909, Sigma).
Hpa011008
Manufactured by Merck Group
The HPA011008 is a laboratory equipment product. It is designed for use in scientific research and analysis. The core function of this product is to perform specific laboratory tasks, but a detailed description cannot be provided while maintaining an unbiased and factual approach. More information may be available from the manufacturer.
Automatically generated - may contain errors
2 protocols using hpa011008
1
Immunofluorescence Staining of Golgi Markers
2
Giantin protein interactome analysis
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The assay was performed using the Duolink kit (Sigma-Aldrich) according to the manufacturer’s protocol. The mouse monoclonal anti-giantin Ab, which recognizes the cytoplasmic N-terminus (3–91 aa) (Sigma, WH0002804M1) was used in combinations with: (a) rabbit polyclonal, which is raised against the central region of giantin (1781–1907 aa) (Sigma, HPA011008); and (b) rabbit polyclonal to giantin (region within N terminal 108–157 aa, Abcam ab93281). After incubation with primary Abs, cells were incubated with oligonucleotide-conjugated anti-mouse minus and anti-rabbit plus proximity ligation assay secondary probes. Subsequent PLA signal was detected by confocal microscopy and the corrected integrated fluorescence intensity was calculated using the ImageJ software.
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