Antibodies against jnk

APAP, N-acetylcysteine (NAC), and other chemicals were delivered by Sigma-Aldrich Chemical Co. (Steinheim, Germany). Salvianolic acid C (Figure 1(a); purify 98.6%) was purchased from Chem Faces Pharmaceutical Company (Wuhan, China). The amounts of mouse TNF-α, IL-1β, and IL-6 ELISA Max™ Set Deluxe Kits were received from BioLegend Inc. (San Diego, CA, US). Primary antibodies against COX-2, p-JNK, catalase, GPx, SOD, Keap1, NQO1, GCLC, and GCLM were purchased from GeneTex (San Antonio, TX, USA). Antibodies against PI3k/p-AKT were purchased from Merck Millipore (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). Antibodies against TLR4, iNOS, NF-κB, IκBα, p38, HO-1, Nrf-2, and β-actin were purchased from Abcam (Cambridge, UK, USA). Antibodies against JNK, p-ERK, ERK, p-p38, and p-IκB-α were purchased from Cell Signaling Technology (Beverly, MA, USA). Protein assay kit (Bio-Rad Laboratories Ltd., Watford, Herts, UK) was obtained as indicated. Poly-(vinylidene fluoride) (PVDF) membrane (Immobilon-P) was obtained from Millipore Corp. (Bedford, MA, USA).

Paracetamol, NAC, other chemicals and solvents were bought from Sigma-Aldrich (St. Louis, MO, USA). ELISA kits for detecting the release of mouse TNF-α, IL-1β, and IL-6 were purchased from BioLegend Inc. (San Diego, CA, USA). Primary antibodies for Western blotting against the proteins COX-2, p-JNK, catalase, GPx, SOD, CYP2E1, AMPK, LKB1, CaMKKβ, p-AMPK, p-LKB1, and p-CaMKKβ were purchased from GeneTex (San Antonio, TX, USA). Antibodies against JNK, p-ERK, ERK, p-p38, and p-IκB-α were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against iNOS, NF-κB, IκBα, p38, HO-1, Nrf-2, and β-actin were purchased from Abcam (Cambridge, UK, USA). Protein assay kits (Bio-Rad Laboratories Ltd., Watford, Herts, UK) were obtained as indicated.