Kaiser test reagents

MDA-MB-231 breast cancer cells were obtained from American Type Culture Collection (CRM-HTB-26). MDA-MB-231 were maintained in complete media consisting of DMEM/F12 (Life Technologies) supplemented with 10% fetal bovine serum (Sigma-Aldrich), 100 IU/ml penicillin and 100 μg/ml streptomycin (Life Technologies). D-glucosamine hydrochloride was purchased from Acros Organics. Kaiser test reagents, cytochalasin B, cytochalasin D and 2-(N-morpholino) ethanesulfonic acid solution (MES, 1 M in water) were obtained from Sigma Aldrich. Short acid oxidized MWCNT (0.5–2 μm in length; 8–15 nm diameter) were obtained from Nanostructured & Amorphous Materials Inc. (TX, USA). N-hydroxysuccinimide ester (NHS) of DSPE-PEG(5000) (DSPE-PEG-NHS) was obtained from Nanocs. 1-ethyl-3-(3-dimethylaminopropyl)carboiimide (EDC) and sulfo-NHS were obtained from Pierce. ³H-glucosamine (specific activity: 37.3 Ci/mmol) was obtained from Perkin Elmer.

We purchased NovaPEG Rink Amide Low Loading Resin, 2-(1H-benzotriazol-1-yl)-1,1,3,3 tetramethyluroniumhexafluorophosphate (HBTU), and all Fmoc-protected amino acids from EMD Millipore. We purchased N,N-diisopropylethylamine (DIEA), trifluoroacetic acid (TFA), triisopropylsilane (TIS), dimethylformamide (DMF), dichloromethane (DCM), 2-proponol (IPA), acetonitrile (AcN), ethanol, Kaiser test reagents, cholesterol, N,N′-diisopropylcarbodiimide (DIC), hydrazine, chloroform, doxorubicin hydrochloride, and DNase I from Sigma-Aldrich. 1,2-ethanedithiol (EDT) was purchased from Alfa Aesar. We obtained 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(glutaryl) (DPPE-GA), 1,2-distearoyl-sn-glycero-3-phosphocholine (sodium salt) (DSPC), and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (mPEG2000-DSPE) from Avanti Polar Lipids. 3H-indolium, 2-(5-(1,3-dihydro-3,3-dimethyl-1-octadecyl-2H-indol-2-ylidene)-1,3-penta-dienyl)-3,3-dimethyl-1-octadecyl-, perchlorate (DiD fluorescent dye) was acquired from Invitrogen. Fmoc-EG₂-OH, Fmoc-EG₆-OH, and Fmoc-EG₈-OH were purchased from Quanta Biodesign. Collagenase type IV was purchased from Life Technologies.