The cells were collected following transfection, and 10 μL of the cell suspension was seeded into a 24-well plate (BD company, USA) at a density of 1.25 × 105/mL. The inoculated cells were placed in an incubator and cultured for 24 h at 37°C in an atmosphere of 5% CO2 [23 (link)]. The cells were stained with the Giemsa kit (Abcam, Shanghai, China). Images of the cells were subsequently captured and the cells were counte.
Giemsa kit
Manufactured by Abcam
Sourced in China
The Giemsa kit is a laboratory reagent used for staining biological samples, particularly blood smears and other cellular preparations, to enhance the contrast and visualization of cellular structures. It provides a standardized staining protocol for the identification and differentiation of various blood cell types.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using giemsa kit
1
Cell Seeding and Giemsa Staining
2
Giemsa Staining of Transfected Cells
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Cells after transfection were washed with PBS for 3 times, and then 10 μL cell
suspension with the density of 1.25 × 105/mL was seeded into the
24-well plate (BD company), the medium containing 10% serum was added into each
well. The inoculated cells were put into the incubator and cultured for 24 h
under the condition of 37 °C and 5% CO2. After that, cells were
stained with Giemsa kit (Abcam). The cells were taken photos and observed.
suspension with the density of 1.25 × 105/mL was seeded into the
24-well plate (BD company), the medium containing 10% serum was added into each
well. The inoculated cells were put into the incubator and cultured for 24 h
under the condition of 37 °C and 5% CO2. After that, cells were
stained with Giemsa kit (Abcam). The cells were taken photos and observed.
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