Igm elisa kit

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom

The IgM ELISA kit is a laboratory tool used to detect and measure the levels of immunoglobulin M (IgM) antibodies in a sample. IgM antibodies are commonly the first to be produced by the immune system in response to an infection or other antigenic stimulus.

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ELISAs (35 citations) IgM Mouse Uncoated ELISA Kit (4 citations) Mouse IgM ELISA Ready-SET-Go kit (3 citations) Human IgM and Human IgG ELISA kits (2 citations) HRP/TMB-based human IgM ELISA Kit (2 citations) Human IgG and IgM ELISA kit (2 citations)

3 protocols using igm elisa kit

Immune Response to 3D-GF-PADM Implant

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This study was approved by the Ethics Committee of Shanghai Ninth People's Hospital, affiliated with the School of Medicine, Shanghai Jiao Tong University. 3D-GF-PADM was implanted subcutaneously into mice (BABL/c, 1 g per kg) for 1 month. CD4⁺ and CD8⁺ cells in the blood were detected by flow cytometry, and the level of IgG/IgM in the blood was detected by an IgG ELISA kit (88-50400-88; eBioscience) and IgM ELISA kit (88-50470; eBioscience).

Liu C, & Sun J. (2020). A porcine acellular dermal matrix induces human fibroblasts to secrete hyaluronic acid by activating JAK2/STAT3 signalling. RSC Advances, 10(32), 18959-18969.

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Quantifying Autoantibody Production In Vitro and In Vivo

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To compare the IgG, IgM Abs production secreted by B cells in vitro, the supernatants were collected from abovementioned systems after 3 days of culture. For the in vivo autoantibody detection, mice were bled at the indicated time points, and sera were collected. IgG, IgM, and anti-dsDNA were, respectively, measured by IgG ELISA kit (eBioscience), IgM ELISA kit (eBioscience), and dsDNA ELISA kit (Alpha Diagnostic, San Antonio, TX, USA). All samples were performed with triplicate. Sera samples were diluted 1/100 for anti-dsDNA and 1/25,000 for measuring IgG.

Zhong H., Liu Y., Xu Z., Liang P., Yang H., Zhang X., Zhao J., Chen J., Fu S., Tang Y., Lv J., Wang J., Olsen N., Xu A, & Zheng S.G. (2018). TGF-β-Induced CD8+CD103+ Regulatory T Cells Show Potent Therapeutic Effect on Chronic Graft-versus-Host Disease Lupus by Suppressing B Cells. Frontiers in Immunology, 9, 35.

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Measurement of Mouse Antibody Levels

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Antibody production in mouse serum was measured by using mouse anti-dsDNA IgG ELISA kit (Chondrex); IgM ELISA kit (eBioscience); IgG ELISA kit (Abcam, Cambridge, England); HRP-conjugated goat anti-mouse IgG1, IgG2b, IgG2c, and IgG3 antibody (Southern Biotech, Birmingham, AL, USA) according to the manufacturer’s instructions. In some experiment, NP-conjugated BSA (Biosearch Technologies, Petaluma, CA, USA) was coated for measuring NP-specific antibody production.

Kim D.H., Park H.J., Park H.S., Lee J.U., Ko C., Gye M.C, & Choi J.M. (2019). Estrogen receptor α in T cells suppresses follicular helper T cell responses and prevents autoimmunity. Experimental & Molecular Medicine, 51(4), 41.

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