Apoptosis was analyzed with an annexin V-fluorescein isothiocyanate (FITC)/7-amino-actinomycin D staining system obtained from BD Biosciences (San Diego, CA). Briefly, prostate cells were harvested and resuspended in binding buffer at a concentration of 1 × 106 cells/ml. For each assay, 1 × 105 cells were incubated with 5 μl of annexin V-FITC and 5 μl of 7-amino-actinomycin D in the dark for 15 min at room temperature. After adding 400 μl of binding buffer, samples were analyzed within an hour by a Cell Lab Quanta™ SC MPL flow cytometer (Beckman Coulter, Fullerton, CA).
Annexin 5 fluorescein isothiocyanate fitc 7 amino actinomycin d staining system
Manufactured by BD
The Annexin V-fluorescein isothiocyanate (FITC)/7-amino-actinomycin D staining system is a laboratory equipment used for the detection and analysis of apoptosis. It consists of Annexin V-FITC, which binds to phosphatidylserine residues exposed on the cell surface during apoptosis, and 7-amino-actinomycin D, which stains dead cells. This system allows for the simultaneous identification of apoptotic and necrotic cells through flow cytometry or fluorescence microscopy.
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2 protocols using annexin 5 fluorescein isothiocyanate fitc 7 amino actinomycin d staining system
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Apoptosis Analysis of Prostate Cells
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Apoptosis Analysis of Prostate Cells
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Apoptosis was analyzed with an annexin V-fluorescein isothiocyanate (FITC)/7-amino-actinomycin D staining system obtained from BD Biosciences (San Diego, CA). Briefly, prostate cells were harvested and resuspended in binding buffer at a concentration of 1 × 106 cells/ml. For each assay, 1 × 105 cells were incubated with 5 μl of annexin V-FITC and 5 μl of 7-amino-actinomycin D in the dark for 15 min at room temperature. After adding 400 μl of binding buffer, samples were analyzed within an hour by a Cell Lab Quanta™ SC MPL flow cytometer (Beckman Coulter, Fullerton, CA).
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