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Total β catenin antibody

Manufactured by Merck Group

The Total β‐catenin antibody is a laboratory reagent used in immunoassays and other experimental techniques to detect and measure the total levels of the β‐catenin protein in biological samples. β‐catenin is a key signaling molecule involved in various cellular processes. This antibody provides a tool for researchers to study the expression and regulation of β‐catenin in their experimental systems.

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2 protocols using total β catenin antibody

1

Methamphetamine-Induced Cellular Senescence

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Methamphetamine (M8750), LiCl, BIO, and propidium iodide were purchased from Sigma (St. Louis, MO). Access to meth was approved by state and federal regulations. Senescence‐associated β‐galactosidase (SA‐βGal) kit and anti‐GFAP G5 antibody were purchased from Cell Signaling Inc (Danvers, MA). Other antibodies include Iba1 antibody (Fisher Scientific #019‐19741, Pittsburgh, PA), active β‐catenin antibody (US Biological C2069‐47, Salem, MA), total β‐catenin antibody (C2206; Sigma), p16INK4A antibody (Proteintech #22515‐1‐AP, Chicago, IL), GAPDH antibody (G99445; Sigma), antineurofilament heavy chain (Millipore Ab5539, Danvers, MA). Click‐iT Plus EdU Alexa Fluor Flow Cytometry Assay kit (C10646) was from Invitrogen (Waltham, MA).
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2

Immunoblotting of Renal β-Catenin

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Immunoblotting was carried out using 10 µg/lane of E13.5 WT and AtminGpg6/Gpg6 whole kidney protein extract (n = at least 9 per genotype run on 3 independent blots); using total β-catenin antibody (Sigma), 1:4000; or non-phospho β-catenin antibody (Cell Signaling Technology), 1:1000. GAPDH (Abcam), 1:5000 was used for loading control.
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