B-Exo were labeled with DiI fluorescent dye (40726ES10; YEASEN Biotech, Shanghai, China) following the manufacturer’s protocol. After removing the redundant dye, B-EVs were added to the cultures of BMSCs or VSMCs and incubated at 37 °C for 3 h. After discarding the culture supernatant and washing the cells with PBS, the cells were fixed in 4% Paraformaldehyde (PFA) for 15 min and incubated with DAPI (D1306; Invitrogen, Carlsbad, USA) to stain nuclei. The uptake of the red DiI-labeled B-EVs by these cells was determined by a Zeiss ApoTome fluorescence microscope (Jena, Germany).
Dii fluorescent dye
Manufactured by Yeasen
Sourced in China
DiI (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate) is a lipophilic carbocyanine dye used for cell labeling and tracing applications. It integrates into cell membranes and is useful for visualizing cellular morphology and tracking cell migration.
Automatically generated - may contain errors
Lab products found in correlation
D1306, by Thermo Fisher Scientific (1 mentions)
Apotome fluorescence microscope, by Zeiss (1 mentions)
Ctr6000, by Leica (1 mentions)
Oil red o staining kit, by Merck Group (1 mentions)
Glycerol, by Merck Group (1 mentions)
Double antibodies, by ScienCell (1 mentions)
Sodium hyaluronate ha, by Merck Group (1 mentions)
High glucose dmem, by ScienCell (1 mentions)
2 protocols using dii fluorescent dye
1
Fluorescent Labeling of B-EVs
2
Aptamer-Mediated Targeted Delivery of Lipid Nanoparticles
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DSPC (Avanti Corporation, USA), DSPE-PEG2000 (Avanti Corporation, USA), DSPE-PEG2000-maleimide (Avanti Company, USA), DC-cholesterol∙HCl (Avanti Company, USA), DPPE (Avanti Company, USA), glycerol (Sigma, USA), sodium hyaluronate (HA, 200–400 kDa, Xi’an Ruixi), aptamer PM1 base sequence SH-CCCCTGCAGGTGATTTTGCTCAAGTCGTTCCCGTCCTCTCCTCTGCGCCCCGTCTCGGCCAGTATCGCTAATCAGGCGGAT-HS, (Shanghai Shenggong, China), a sequence with FITC-labelled or unlabelled at the 5ʹ end, ANM33 and ANM33 double-modified with cy3 and amino groups (Guangzhou Ruibo, China), DiI fluorescent dye (Yeasen, USA), RAW264.7-T1 macrophages (Procell Life Science & Technology, China), oxidized low-density lipoprotein (ox-LDL; Yiyuan Biotechnology, China), Oil red O staining kit (Sigma, China), high-glucose DMEM (ScienCell, China), and double antibodies (ScienCell, USA) were used. A fluorescence microscope (LEICA CTR6000, Germany), laser confocal microscope (Leica SP8, Germany), flow cytometer (Beckman, USA), and laser nanoparticle size and zeta potential analyser (Malvern, UK) were also used.
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