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Il 2 bv605 clone mq1 17h12

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IL-2-BV605 (clone MQ1–17H12) is a fluorescent-labeled antibody that binds to the cytokine interleukin-2 (IL-2). It is designed for use in flow cytometry and other immunoassay applications to detect and quantify IL-2 in biological samples.

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Lab products found in correlation

Golgistop protein transport inhibitor, by BD (2 mentions) Facsdiva software package, by BD (2 mentions) Ifn γ pe clone b7, by BD (2 mentions) Tnf α af700 clone mab11, by BD (2 mentions) Lsrii flow cytometer, by BD (2 mentions)

2 protocols using il 2 bv605 clone mq1 17h12

1

Dissecting SIV-Specific T-Cell Responses

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PBMCs from five ART-suppressed SIV-infected RMs were thawed. Pretreatment with AZD5582 was performed for 1 h at 100 nm. After two washes, PMA and ionomycin were added for 1 h at 500 ng/mL and 10 μg/mL, respectively. DMSO treatment controls were prepared in parallel. After 1 h, brefeldin-A (BFA) and Golgi stop solution were added following manufacturer’s recommendations (BD GolgiStop™ Protein Transport Inhibitor, BD Biosciences). Cells were incubated at 37°C, 5% CO2 in R10 for 6–8 h before staining with the following antibodies: CD3-APC-Cy7 (clone SP34–2), CD8-BV711 (clone RPA-T8), CD4-BV650 (clone OKT4), from Biolegend, IFN-γ-PE (clone B7), TNF-α-AF700 (clone Mab11) and IL-2-BV605 (clone MQ1–17H12) from BD Biosciences. Flow cytometric acquisition and analysis of samples was performed on at least 100,000 events on an LSRII flow cytometer driven by the FACSDiva software package (BD Biosciences). Analyses of the acquired data were performed using FlowJo™ (TreeStar, version 10.0.4) and Simplified Presentation of Incredibly Complex Evaluations (SPICE, version 6.0) software.
Nixon C.C., Mavigner M., Sampey G.C., Brooks A.D., Spagnuolo R.A., Irlbeck D.M., Mattingly C., Ho P.T., Schoof N., Cammon C.G., Tharp G.K., Kanke M., Wang Z., Cleary R.A., Upadhyay A.A., De C., Wills S.R., Falcinelli S.D., Galardi C., Walum H., Schramm N.J., Deutsch J., Lifson J.D., Fennessey C.M., Keele B.F., Jean S., Maguire S., Liao B., Browne E.P., Ferris R.G., Brehm J.H., Favre D., Vanderford T.H., Bosinger S.E., Jones C.D., Routy J.P., Archin N.M., Margolis D.M., Wahl A., Dunham R.M., Silvestri G., Chahroudi A, & Garcia J.V. (2020). Systemic HIV and SIV latency reversal via non-canonical NF-κB signalling in vivo. Nature, 578(7793), 160-165.
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2

Dissecting SIV-Specific T-Cell Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs from five ART-suppressed SIV-infected RMs were thawed. Pretreatment with AZD5582 was performed for 1 h at 100 nm. After two washes, PMA and ionomycin were added for 1 h at 500 ng/mL and 10 μg/mL, respectively. DMSO treatment controls were prepared in parallel. After 1 h, brefeldin-A (BFA) and Golgi stop solution were added following manufacturer’s recommendations (BD GolgiStop™ Protein Transport Inhibitor, BD Biosciences). Cells were incubated at 37°C, 5% CO2 in R10 for 6–8 h before staining with the following antibodies: CD3-APC-Cy7 (clone SP34–2), CD8-BV711 (clone RPA-T8), CD4-BV650 (clone OKT4), from Biolegend, IFN-γ-PE (clone B7), TNF-α-AF700 (clone Mab11) and IL-2-BV605 (clone MQ1–17H12) from BD Biosciences. Flow cytometric acquisition and analysis of samples was performed on at least 100,000 events on an LSRII flow cytometer driven by the FACSDiva software package (BD Biosciences). Analyses of the acquired data were performed using FlowJo™ (TreeStar, version 10.0.4) and Simplified Presentation of Incredibly Complex Evaluations (SPICE, version 6.0) software.
Nixon C.C., Mavigner M., Sampey G.C., Brooks A.D., Spagnuolo R.A., Irlbeck D.M., Mattingly C., Ho P.T., Schoof N., Cammon C.G., Tharp G.K., Kanke M., Wang Z., Cleary R.A., Upadhyay A.A., De C., Wills S.R., Falcinelli S.D., Galardi C., Walum H., Schramm N.J., Deutsch J., Lifson J.D., Fennessey C.M., Keele B.F., Jean S., Maguire S., Liao B., Browne E.P., Ferris R.G., Brehm J.H., Favre D., Vanderford T.H., Bosinger S.E., Jones C.D., Routy J.P., Archin N.M., Margolis D.M., Wahl A., Dunham R.M., Silvestri G., Chahroudi A, & Garcia J.V. (2020). Systemic HIV and SIV latency reversal via non-canonical NF-κB signalling in vivo. Nature, 578(7793), 160-165.
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