We drew blood samples from a brachial vein in all patients upon admission. Blood was collected in EDTA tubes. After centrifuged (3000 g/min for 10 min), plasma and plasma aliquots were stored at −80°C freezer. Upon thawing from the freezer, samples were applied to experiments immediately. The D-dimer was measured by the human D-dimer ELISA kit (ab196269, Abcam, Cambridge, MA, USA) with a sensitivity of 71 pg/mL. We quantified the ET-1 level by an Endothelin-1 ELISA Kit (ab133030, Abcam, Cambridge, MA, USA) with a sensitivity of 0.41 pg/mL. The C-reactive protein, creatine kinase (CK), CK-MB, and troponin T plasma levels were measured every 4 hours during the first day and every 24 hours in the following 3 days using routine methods. The LV ejection fraction was quantified within 2 hours after the PCI procedure by 2D-echocardiography (Simpson method).
Endothelin 1 elisa kit
Manufactured by Abcam
Sourced in United States, United Kingdom
The Endothelin-1 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of Endothelin-1 levels in biological samples.
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Lab products found in correlation
2 protocols using endothelin 1 elisa kit
1
Biomarkers and Cardiac Function Assessment
2
Serum Biomarkers in Post-Operative Rats
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Four weeks after the operation, 10 mL fasting venous blood of rats were taken from the right carotid artery under the aseptic condition. The serum level of B-type natriuretic peptide (BNP) was measured using a BNP ELISA Kit (ab108815; Abcam, Cambridge, UK) and that of endothelin 1 was measured using an endothelin 1 ELISA Kit (ab133030; Abcam) based on the manufacturer's instructions.
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