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4 protocols using nacl salt

1

Biogenic Synthesis of Iron Oxide Nanoparticles

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Methyl green dye, NaCl salt, and HCl acid were purchased from Sigma-Aldrich. JUN-Fe3O4, ROS-Fe3O4, MAT-Fe3O4 and ARM-Fe3O4 NP powders were greenly synthesised using an iron salt (FeCl3·6H2O) (purchased from Biochem Chemopharma Co, Canada) as the precursor and Artemisia herba-alba (L) (Asteraceae family), Matricaria pubescens (L) (Asteraceae family), Juniperus phoenicia (L) (Cupressaceae family), Rosmarinus officinalis (L) (Lamiaceae family) plants as reducing agents. Fe3O4 samples were obtained after 4 months of storage of the synthesized iron oxides in ambient conditions. The freshly synthesized samples were both wüstite and magnetite.67 (link) The chemical structure of MG is presented in Fig. 1.
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2

Greenly Synthesized Magnetic Nanoparticles

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Evans blue, methyl orange dyes, NaCl salt, HCl acid, and NaOH base are purchased from Sigma-Aldrich. JUN–Fe3O4, MAT–Fe3O4, ROS–Fe3O4 and ARM–Fe3O4 nanoparticle powders were greenly synthesised using iron salt (FeCl3·6H2O) (purchased from Biochem Chemopharma Co, Canada) as precursor and Artemisia herba-alba (L) (Asteraceae family), Matricaria pubescens (L) (Asteraceae family), Juniperus phoenicea (L) (Cupressaceae family), and Rosmarinus officinalis (L) (Lamiaceae family) plants as reducing agents. Magnetite samples were obtained after 4 months of storage of the synthesized iron oxides under ambient conditions. Characterization of the freshly synthesized iron oxides samples is presented in Ahmouda et al.38 (link)
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3

Biofunctionalized Surfaces for Biosensing

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Poly-l-lysine hydrobromide (MW =
15–30 kDa by viscosity), EDC, NHS, tablets for 10 mM PBS solution
(pH 7.4), 3-trichlorosilylpropyl methacrylate, 2-hydroxyethyl methacrylate
(HEMA), ethylene glycoldimethacrylate (EGDMA), tridecafluoro-(1,1,2,2)-tetrahydrooctyl-trichlorosilane,
bovine serum albumin, ammonium persulfate (APS), and NaCl salt were
purchased from Sigma-Aldrich. Methyl-OEG4-NHS ester, biotin-OEG4-NHS ester, Mal-OEG4-NHS ester, the Zeba spin desalting
columns (7 kDa MWCO, 5 mL), the immobilized TCEP disulfide reducing
gel (tris[2-carboxyethyl] phospine hydrochloride immobilized onto
4%cross-linked beaded agarose), and the streptavidin-Fluorescein conjugate
were obtained from ThermoFisher Scientific. Oligonucleotides were
purchased from Eurofins Genomics and used as received. SiO2 QCM chips (with fundamental frequency of 5 MHz) were purchased from
Biolin Scientific. Poly(dimethylsiloxane) Sylgard 184 and curing agent
were used as received from Dow Corning. Ormostamp material and MA-T1050
were purchased from Microresist. The PNA probes were synthesized using
the materials and the procedure previously described for PNA2,52 (link) as briefly reported in the Supporting Information together with the characterization
of the newly synthesized PNA3 and PNA4.
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4

Synthesis and Characterization of Emissive AgNCs

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DNA/RNA chimera probes and desalted miRNA targets were obtained from IDT (Integrated DNA Technologies, BVBA. Interleuvenlaan 12A, 3001 Leuven, Belgium). The synthesis of emissive AgNCs was carried out using AgNO 3 (99.9999%) and NaBH 4 (99.99%) from Sigma Aldrich. Tris-acetate buffer ( pH 7, 0.5 M) was prepared with TRIZMA® acetate salt (≥99.0%, from Sigma Aldrich) in pure Milli-Q water (18.2 MΩ cm). A NaCl (5 mM) solution was prepared with NaCl salt (≥98.0%, from Sigma Aldrich) in pure Milli-Q water.
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