5 v1 gem beads

Manufactured by 10x Genomics

Sourced in United States

The 5' v1 Gem beads are a key component in the 10x Genomics' single-cell RNA sequencing workflow. These beads are designed to capture and label individual cells, enabling high-throughput analysis of gene expression profiles at the single-cell level.

Automatically generated - may contain errors

Lab products found in correlation

Nextseq 550, by Illumina (2 mentions) Cfx384, by Bio-Rad (1 mentions)

Spelling variants of this product

Chromium Next GEM Single Cell 5’ Library & Gel Bead Kit v1.1 (5 citations) Chromium Next GEM Single Cell 5′ Library and Gel Bead Kit v1.1 (5 citations) Chromium Next GEM Single Cell 5' Library & Gel Bead Kit v1.1 or v2 (2 citations)

2 protocols using 5 v1 gem beads

Multiplexed Single-Cell Immune Profiling

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Single-cell encapsulations were generated using 5’ v1 Gem beads from 10x Genomics (Pleasanton, California, USA) on a 10x Chromium controller and downstream TCR, and Surface marker libraries were made following manufacturer recommended conditions. All libraries were quantified on a BioRad CFX 384 (Hercules, California, USA) using Kapa Biosystems (Wilmington, Massachusetts, USA) library quantified kits and pooled at an equimolar ratio. TCRs, surface markers, and tetramer-generated libraries were sequenced on Illumina (San Diego, California, USA) NextSeq 550 instruments. Sequencing data were processed using the Cell Ranger Software Suite (V.3). Samples were demultiplexed and unique molecular identifier (UMI) counts were quantified for TCRs, tetramers, and gene expression.

McInnis C., Bhatia S., Vijaykumar B., Tian Q., Sun Y., Leistritz-Edwards D., Quinn C.T., Uppaluri R., Egloff A.M., Srinivasan L., Pregibon D.C., Coyle A.J, & Hanna G.J. (2023). Identification of HPV16 E1 and E2-specific T cells in the oropharyngeal cancer tumor microenvironment. Journal for Immunotherapy of Cancer, 11(3), e006721.

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Single-cell TCR and Surface Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tetramer positive cells were counted by Nexcelom Cellometer (Lawrence, MA, USA) using AOPI stain following manufacturer’s recommended conditions. Single-cell encapsulations were generated utilizing 5′ v1 Gem beads from 10x Genomics (Pleasanton, CA, USA) on a 10x Chromium controller and downstream TCR, and Surface marker libraries were made following manufacturer recommended conditions. All libraries were quantified on a BioRad CFX 384 (Hercules, CA, USA) using Kapa Biosystems (Wilmington, MA, USA) library quantified kits and pooled at an equimolar ratio. TCRs, surface markers, and tetramer generated libraries were sequenced on Illumina (San Diego, CA, USA) NextSeq550 instruments.

Weingarten-Gabbay S., Klaeger S., Sarkizova S., Pearlman L.R., Chen D.Y., Gallagher K.M., Bauer M.R., Taylor H.B., Dunn W.A., Tarr C., Sidney J., Rachimi S., Conway H.L., Katsis K., Wang Y., Leistritz-Edwards D., Durkin M.R., Tomkins-Tinch C.H., Finkel Y., Nachshon A., Gentili M., Rivera K.D., Carulli I.P., Chea V.A., Chandrashekar A., Bozkus C.C., Carrington M., Bhardwaj N., Barouch D.H., Sette A., Maus M.V., Rice C.M., Clauser K.R., Keskin D.B., Pregibon D.C., Hacohen N., Carr S.A., Abelin J.G., Saeed M, & Sabeti P.C. (2021). Profiling SARS-CoV-2 HLA-I peptidome reveals T cell epitopes from out-of-frame ORFs. Cell, 184(15), 3962-3980.e17.

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