Vegf enzyme linked immunosorbent assay elisa kit

Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from ATCC (Manassas, VA). Medium 200 (M200), low serum growth supplement (LSGS), bovine serum albumin (BSA), fetal bovine serum (FBS), Geltrex, and calcein AM were purchased from ThermoFisher (Waltham, MA). VEGF-165 (VEGF, MW= 38.2 kDa) and VEGF enzyme-linked immunosorbent assay (ELISA) kit were obtained from Peprotech (Rocky Hill, NJ). CellTiter-Glo^® Luminescent Cell Viability Assay (ATP) were obtained from Promega (Madison, WI). Acrydite modified VEGF (MW ~10 kDa) and c-MET (MW ~17.5 kDa) aptamers, and their complementary sequence (CS) were custom ordered from Integrated DNA technologies (Coralville, PA).

Calcein AM, glycine, bovine serum albumin (BSA), fetal bovine serum (FBS), medium 200 (M200), medium 106 (M106), medium 154 (M154), Low Serum Growth Supplement (LSGS), ABTS substrate, xylene substitute mountant, ProLong Diamond Antifade Mountant with DAPI, goat anti-rabbit IgG-Alexa Fluor 546 antibody, α-smooth muscle actin antibody-Alexa Fluor 488 antibody (α-SMA), and Human umbilical vein endothelial cells (HUVECs), were obtained from Thermo-Fisher Scientific. Nucleic acid sequences were obtained from Integrated DNA Technologies (Coralville, IA). Vascular endothelial growth factor-165 (VEGF) and VEGF enzyme-linked immunosorbent assay (ELISA) kit were obtained from Peprotech (Rocky Hill, NJ). 4% Paraformaldehyde was obtained from Santa Cruz (Dallas, TX). Primary rabbit anti-mouse CD31 antibody was obtained from Cell Signaling Technology (Beverly, MA). Human neonatal fibroblasts (HDFN) and mouse keratinocytes (MK) were obtained from ATCC (Manassas, VA). Hematoxylin and eosin (H&E) stain kit was obtained from Leica Biosystems (Buffalo Grove, IL). Collagenase P. was obtained from Sigma Aldrich (St. Louis, MO).