Tumor necrosis factor α tnf α elisa kit

HUVECs (Jennio, China), Dulbecco modified eagle medium (DMEM) with low sugar (Hyclone, USA), fetal serum bovine (FBS, BI, India), penicillin-streptomycin (Bi YT, China), 0.25% pancreatin (Bi YT, China), rhACE2 (R&D Systems, USA), Ang II (Torics, UK), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyletrazolium bromide (MTT) cell proliferation and cytotoxicity assay kit (Sigma, USA), propidium iodide/ribonuclease (PI/RNase) cycle detection kit (BD, USA), fluorescein isothiocyanate (FITC) annexin V apoptosis detection kit 1 (BD, USA), reactive oxygen species (ROS) detection kit (Bi YT, China), interleukin (IL)-8 enzyme-linked immunosorbent assay (ELISA) kit (R&D, USA), tumor necrosis factor α (TNF-α) ELISA kit (R&D, USA), transforming growth factor-β1 (TGF-β1) ELISA kit (Abcam, USA), lactate dehydrogenase (LDH) ELISA kit (Jiancheng, China). HF90/HF240 cell incubator (Heal Force, China), Multiskan GO 1510 spectrophotometer (Thermo Fisher, USA), BD FACS Aria III flow cytometer (BD, USA).

LPS (Escherichia coli, 0111:B4) was purchased from Sigma (St. Louis, MO, USA). RvD1 was obtained from Cayman (Ann Arbor, MI, USA). Tert-butoxycarbonyl Met-Leu-Phe peptide (Boc-MLP, RvD1 receptor blocker), anti-phosphorylated-IκB alpha (rabbit monoclonal), anti-IκB alpha (rabbit monoclonal), and anti-lipoxin A4 receptor (ALX, RvD1 receptor, and rabbit polyclonal) were from Santa Cruz (Dallas, TX, USA). Anti-cleaved caspase-3 (rabbit polyclonal) was obtained from Abcam (Cambridge, UK). The real-time polymerase chain reaction (PCR) kit was purchased from Takara Bio (Shiga, Japan). In situ cell death fluorescein (TUNEL) kit was purchased from Roche (Basel, Switzerland). Tumor necrosis factor-α (TNF-α) ELISA kit was from R&D (Minneapolis, MN, USA).