P anisaldehyde h2so4 reagent

The UV and IR spectra were recorded on UV-1601-PC (Shimadzo, Koyoto, Japan) and JASCO 320-A spectrometers (JASCO Germany GmbH, Gross-Umstadt, Germany). The ¹H-, ¹³C-NMR and 2D-NMR spectra were recorded on a Bruker AMX-500 spectrometer (Bruker, Faellanden, Switzerland) with tetramethylsilane (TMS) as internal standard. Chemical shifts are given in ppm (δ) relative to tetramethylsilane internal standard and scalar coupling constants (J) are reported in Hertz. Jeol JMS-700 High Resolution Mass Spectrophotometer (JEOL (Germany) GmbH, Muenchen, Germany) was used for the mass determination. Electron Impact mode of ionization was used, keeping ionization energy of 70 eV. Resolution was set up to 10 k. A direct probe was used with temperature ramp setting, initial temperature 50 °C rise with rate of 32 °C per minute and final temperature set up to 350 °C. Thin layer chromatography (TLC) was performed on precoated silica gel F₂₅₄ plates (E. Merck, Darmstadt, Germany); detection was done at 254 nm and by spraying with p-anisaldehyde/H₂SO₄ reagent. All chemicals were purchased from Sigma Chemical Company (St. Louis, MO, USA).