RIP assay was performed using an EZ-Magna RiP Kit (Millipore, Billerica, MA, USA), in accordance with the manufacturer’s instructions. Cells were lysed at 70%–80% confluence in RIP lysis buffer, and then they were incubated with magnetic beads conjugated with human anti-Ago2 antibody (Millipore) and normal mouse immunoglobulin G (IgG) control (Millipore) in RIP buffer. The RNAs in the immunoprecipitates were isolated with Trizol reagent and analyzed by qRT-PCR.
Normal mouse immunoglobulin g igg control
Manufactured by Merck Group
Normal mouse immunoglobulin G (IgG) control is a laboratory reagent used as a reference or control in immunological assays. It provides a source of mouse IgG, which is the most abundant antibody class in the serum of mice.
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Lab products found in correlation
4 protocols using normal mouse immunoglobulin g igg control
1
Ago2-mediated RNA Immunoprecipitation Assay
2
Ago2-RIP Assay Protocol
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RIP assay was performed using an EZ-Magna RiP Kit (Millipore, Billerica, MA, USA), in accordance with the manufacturer’s instructions. Cells were lysed at 70%–80% confluence in RIP lysis buffer, and then they were incubated with magnetic beads conjugated with human anti-Ago2 antibody (Millipore) and normal mouse immunoglobulin G (IgG) control (Millipore) in RIP buffer. The RNAs in the immunoprecipitates were isolated with Trizol reagent and analyzed by qRT-PCR.
3
RNA Immunoprecipitation Assay Protocol
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The RIP assay was performed using an EZ-Magna RiP Kit (Millipore, Billerica, MA, USA) in accordance with the manufacturer’s instructions. Cells were lysed at 70%–80% confluence in RIP lysis buffer and then incubated with magnetic beads conjugated with human anti-Ago2 antibody (Millipore) and normal mouse immunoglobulin G (IgG) control (Millipore) in RIP buffer. The RNAs in the immunoprecipitates were isolated with TRIzol Reagent and analyzed by quantitative real-time PCR.
4
RNA Immunoprecipitation Assay for AGO2
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RIP assay was performed using an EZ-Magna RIP kit (Millipore, Billerica, MA, USA) in accordance with the manufacturer’s instructions. Cells were lysed at 70%–80% confluence in RIP lysis buffer and then incubated with magnetic beads conjugated with human anti-AGO2 antibody (Millipore) and normal mouse immunoglobulin G (IgG) control (Millipore) in RIP buffer. The RNAs in the immunoprecipitates were isolated with TRIzol reagent and analyzed by qRT-PCR.
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