Cell proliferation was measured using the CellTiter 96 Non-radiactive Cell Proliferation Assay kit (Promega). LL/2-luc-M38 cells (3 × 104/well) were seeded into 96-well plates in six replicates. Cell proliferation rates were determined on five consecutive days using the automated microtiter plate reader Power Wave WS (BioTek).
Celltiter 96 non radiactive cell proliferation assay kit
Manufactured by Promega
The CellTiter 96 Non-radiactive Cell Proliferation Assay kit is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. The assay is based on the cellular conversion of a tetrazolium salt into a colored formazan product.
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3 protocols using celltiter 96 non radiactive cell proliferation assay kit
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Cell Proliferation Assay Protocol
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CellTiter 96 Cell Proliferation Assay
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Cell proliferation was measured using the CellTiter 96 Non-radiactive Cell Proliferation Assay kit purchased from Promega. 103 cells were seeded in 96-well plates and six replicates per condition and time point were assessed. Cell proliferation rates were determined on three (MCF-7) or four (MDA-MB-231) consecutive days using an automated microtiter plate reader Power Wave WS (BioTek).
3
CellTiter 96 Cell Proliferation Assay
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Cell proliferation was measured using the CellTiter 96 Non-radiactive Cell Proliferation Assay kit (Promega). MBA-MB-231 and 4T1 cells (5×10 3 /well) were seeded in 96-well plates and six replicates were performed per condition. Cell proliferation rates were determined on four (4T1) or five (MDA-MB-231) consecutive days using an automated microtiter plate reader Power Wave WS (BioTek). Additionally, cell proliferation was also estimated as an average of Ki-67-positive nuclei in relation to the total number of nuclei per microscopic field as previously reported [19] . To this end, 5×10 3 stably transfected cells were plated in µ-Dish 35 mm from Ibidi.
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