7 350 auto analyzer

Venous blood samples were obtained in the morning following an overnight fast. Serum lipids were measured using a Hitachi 7,350 auto analyzer (Hitachi, Tokyo, Japan). Low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglyceride (TG) were determined by the enzymatic method, and LDL-C was determined by the direct method. Fasting plasma glucose levels were measured using a standard enzymatic method. Hemoglobin A1c (HbA1c) (%) was measured by high-performance liquid chromatography using a Tosoh HLC-723 G8 (Tosoh, Kyoto, Japan) and expressed as National Glycohemoglobin Standardization Program (NGSP) values by adding 0.4% to the HbA1c values, which were expressed as conventional Japanese standard substance (JDS) values (15 ). The homeostasis model assessment of insulin resistance (HOMA-IR) was calculated as follows: fasting plasma glucose (FPG) (mg/dL) × fasting plasma insulin (FPI) (μU/mL) / 405. Anti-cyclic citrullinated peptide antibody was measured using a chemiluminescence enzyme immunoassay (CLEIA) (STACIA System; LSI Medience Corporation, Tokyo, Japan). Matrix metalloproteinase-3 was measured using a latex turbidimetric immunoassay (LTIA) (JCA-BM8000 series; JEOL, Tokyo, Japan). Pentraxin-3 was measured using an enzyme-linked immunosorbent assay (ELISA) (human pentraxin-3 ELISA System; Perseus Proteomics, Tokyo, Japan).

Blood samples were collected early in the morning after at least 12-h fasting, through a venous line placed in the median vein using an indwelling catheter. PG level was measured with the glucose oxidase method. HbA1c was measured via high-performance liquid chromatography (HPLC) using a Tosoh HLC-723G8 (Tosoh, Kyoto, Japan). HbA1c (%) was estimated as the National Glycohemoglobin Standardization Program (NGSP) equivalent value, which was calculated as HbA1c (NGSP) (%) = HbA1c (JDS) (%) + 0.4%, considering the relationship of HbA1c (NGSP) values to HbA1c (JDS) (%) values determined by the Japanese standard and measurement method. HOMA-IR, which represents insulin resistance, was calculated using the following formula: HOMA-IR = Fasting glucose level × Fasting insulin level ÷ 405. Measurement of lipid profiles and other markers was outsourced to SRL Co. (Tokyo, Japan). Plasma lipid was measured with a Hitachi 7,350 autoanalyzer (Hitachi, Tokyo, Japan). LDL-C was measured using the colestest LDL (Sekisui Medical, Tokyo, Japan) by the direct method. HDL-C was measured using the colestest NHDL (Sekisui Medical) by the direct method. TG was measured using the pureanto STG-N (Sekisui Medical) by the enzymatic method. HMW-AN was measured via a chemiluminescent enzyme immunoassay. u-C-peptide reactivity (CPR) level was measured in 24-h urine samples.