Sample collection and western blotting were performed as previously reported36 (link),47 (link) with the following primary antibodies: anti-VEGFA antibody (Abcam), mouse monoclonal anti-α-SMA (Sigma-Aldrich), rabbit monoclonal anti-TGF-β1 (Abcam), IDO1 polyclonal antibody (Proteintech, Rosemont, IL, USA), mouse monoclonal anti-Foxp3 (Abcam), rabbit polyclonal anti-CD4 (Abcam), and mouse monoclonal anti-GAPDH (Sigma-Aldrich). Horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (Dako) or goat anti-mouse immunoglobulin G (Dako) were used as secondary antibodies. SuperSignal West Dura or Pico Systems (Thermo Fisher Scientific, Waltham, MA, USA) were used to detect signals. The intensity of each band was analyzed by ImageJ software and standardized to the level of GAPDH.
Mouse monoclonal anti foxp3
Manufactured by Abcam
Sourced in United States, United Kingdom
Mouse monoclonal anti-Foxp3 is a laboratory reagent that can be used to detect the Foxp3 protein. Foxp3 is a transcription factor that is expressed in T regulatory cells and plays a role in their development and function.
Automatically generated - may contain errors
Lab products found in correlation
Anti vegfa antibody, by Abcam (1 mentions)
Ido1 polyclonal antibody, by Proteintech (1 mentions)
Horseradish peroxidase conjugated goat anti rabbit immunoglobulin g, by Agilent Technologies (1 mentions)
Goat anti mouse immunoglobulin g, by Agilent Technologies (1 mentions)
Supersignal west dura, by Thermo Fisher Scientific (1 mentions)
Mouse monoclonal anti gapdh, by Merck Group (1 mentions)
Mouse monoclonal anti α sma, by Merck Group (1 mentions)
Monoclonal rabbit anti tgf β1, by Abcam (1 mentions)
Rabbit polyclonal anti collagen type 1, by Abcam (1 mentions)
Rabbit polyclonal anti cd3, by Agilent Technologies (1 mentions)
2 protocols using mouse monoclonal anti foxp3
1
Quantitative Protein Expression Analysis
2
Quantifying Immune Cell Populations in Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Immunohistochemical staining was performed according to previously described methods47 (link) using the following primary antibodies: mouse monoclonal anti-Foxp3 (Abcam, Cambridge, UK), rabbit polyclonal anti-CD3 (Dako, Glostrup, Denmark), mouse monoclonal anti-rat CD68 (Serotec, Oxford, UK), and rabbit polyclonal anti-collagen type I (Abcam). FOXP3-, CD3- and CD68-positive cells, as well as areas positive for α-SMA and collagen type I staining, were assessed using ImageJ software (version 1.53 s, NIH) by examining five randomly selected fields (100× magnification) of the cortex.
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