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Andphospho cofilin

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Andphospho-COFILIN is a laboratory product that detects phosphorylated forms of the COFILIN protein. COFILIN is an actin-binding protein involved in the regulation of the actin cytoskeleton. Phosphorylation of COFILIN is a key mechanism that modulates its activity and function.

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Lab products found in correlation

C300 imaging system, by Azure Biosystems (2 mentions) Phospho mlc2 ser19, by Cell Signaling Technology (2 mentions) Cofilin, by Cell Signaling Technology (2 mentions) β actin, by Merck Group (2 mentions)

Spelling variants of this product

Cofilin (77 citations) Andphospho-COFILIN Ser3 (2 citations)

2 protocols using andphospho cofilin

1

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were lysed in RIPA buffer [50mM Tris-HCl (pH 7.4), 150mM KCl,
1mM EDTA, 1% Triton X-100, 0.5% Sodium deoxycholate, protease and phosphatase
inhibitors]. Proteins were separated by SDS-PAGE and transferred onto
nitrocellulose membranes. Blocking and antibody incubations were performed in 5%
BSA. Myosin light chain 2 (MLC2) (Cat#:3672, 1:1000 dilution), phospho-MLC2
(Ser19) (Cat#:3671, 1:1000 dilution), COFILIN (Cat#:5175, 1:1000 dilution) and
phospho-COFILIN (Cat#:3311, 1:1000 dilution) antibodies were purchased from Cell
Signaling; beta-ACTIN (Sigma-Aldrich, Cat#:A5441, 1:1000 dilution) antibody
detection was used as loading control. Antibody detection reactions were
developed by enhanced chemiluminescence (Advansta) and imaged using the c300
imaging system (Azure Biosystems). Quantification was done using ImageJ
software.
Perino A., Velázquez-Villegas L.A., Bresciani N., Sun Y., Huang Q., Fénelon V.S., Castellanos-Jankiewicz A., Zizzari P., Bruschetta G., Jin S., Baleisyte A., Gioiello A., Pellicciari R., Ivanisevic J., Schneider B.L., Diano S., Cota D, & Schoonjans K. (2021). Central anorexigenic actions of bile acids are mediated by TGR5. Nature metabolism, 3(5), 595-603.
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2

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Samples were lysed in RIPA buffer [50mM Tris-HCl (pH 7.4), 150mM KCl,
1mM EDTA, 1% Triton X-100, 0.5% Sodium deoxycholate, protease and phosphatase
inhibitors]. Proteins were separated by SDS-PAGE and transferred onto
nitrocellulose membranes. Blocking and antibody incubations were performed in 5%
BSA. Myosin light chain 2 (MLC2) (Cat#:3672, 1:1000 dilution), phospho-MLC2
(Ser19) (Cat#:3671, 1:1000 dilution), COFILIN (Cat#:5175, 1:1000 dilution) and
phospho-COFILIN (Cat#:3311, 1:1000 dilution) antibodies were purchased from Cell
Signaling; beta-ACTIN (Sigma-Aldrich, Cat#:A5441, 1:1000 dilution) antibody
detection was used as loading control. Antibody detection reactions were
developed by enhanced chemiluminescence (Advansta) and imaged using the c300
imaging system (Azure Biosystems). Quantification was done using ImageJ
software.
Perino A., Velázquez-Villegas L.A., Bresciani N., Sun Y., Huang Q., Fénelon V.S., Castellanos-Jankiewicz A., Zizzari P., Bruschetta G., Jin S., Baleisyte A., Gioiello A., Pellicciari R., Ivanisevic J., Schneider B.L., Diano S., Cota D, & Schoonjans K. (2021). Central anorexigenic actions of bile acids are mediated by TGR5. Nature metabolism, 3(5), 595-603.
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