Cobas hdlc3

Plasma glucose concentrations were determined twice by the glucose oxidase method using a Beckman glucose analyzer II (Beckman Instruments, Brea, CA, USA). Total plasma cholesterol, HDL-C, and total plasma triglycerides were measured the cholesterol esterase–cholesterol oxidase–peroxidase reaction (Cobas CHOL2, Roche, Basel, Switzerland), the cholesterol esterase–cholesterol oxidase–peroxidase reaction (Cobas HDLC3, Roche, Basel, Switzerland), and the glycerol phosphate oxidase and peroxidase (Cobas TRIGL, Roche, Basel, Switzerland) enzymatic colorimetric methods, respectively.

For the rat samples, plasma levels of T₃ and T₄ were measured using rat ELISA kits (Crystal Chem Inc; Downers Grove, IL, USA) (Lopez et al. 2010 (link), Gonzalez et al. 2012 (link), Varela et al. 2012 (link)). For the human samples, serum glucose concentrations were measured in duplicate by the glucose oxidase method using a Beckman Glucose Analyser II (Beckman Instruments; Brea, CA, USA). Roche Hitachi Cobas c711 instrument (Roche) was used to perform HDL cholesterol and total serum triglycerides determinations. HDL cholesterol was quantified by a homogeneous enzymatic colorimetric assay through the cholesterol esterase/cholesterol oxidase/peroxidase reaction (Cobas HDLC3; Roche). Serum fasting triglycerides were measured by an enzymatic, colorimetric method with glycerol phosphate oxidase and peroxidase (Cobas TRIGL; Roche). LDL cholesterol was calculated using the Friedewald formula. Serum free T₄ was measured by electrochemiluminescence (Roche Diagnostics) with intra- and inter-assay coefficients of variation less than 5%. Methods have been previously reported (Ortega et al. 2015 (link), Gavalda-Navarro et al. 2016 (link)).