Betasil phenyl hplc column

HPLC was performed with Shimadzu LC 10AS solvent delivery system equipped with UV/VIS variable wavelength detector Shimadzu SPD 10A (Shimadzu Corporation, Japan) and an integrator Shimadzu C-R8A (Shimadzu Corporation, Japan). Chromatographic resolution of components in tea was achieved on a 2.1 × 150 mm, betasil phenyl HPLC column (Thermo scientific). The temperature of the chromatographic column was maintained at 25 °C throughout all experiments. Samples were injected with a syringe loading injector fitted with a 100 μL loop. Shimadzu Libror AEG-220 analytical balance (Shimadzu Corporation, Japan) was used to prepare standard solutions. Purified deionized water was obtained from Labconco Water Pro-PS UV ultra filtered water system (Labconco Corporation, Missouri). Micro-centrifugation was performed using a BioFuge-Pico D-37520 centrifuge (Heraeus Instruments, Germany).

Shimadzu UV-1601 UV Visible spectrophotometer (Shimadzu Corporation, Japan) was used to read the absorbance. HPLC was performed with Shimadzu LC 10AS solvent delivery system equipped with UV/VIS variable wavelength detector Shimadzu SPD 10A (Shimadzu Corporation, Japan) and an integrator Shimadzu C-R8A (Shimadzu Corporation, Japan). Chromatographic resolution of components in tea was achieved on betasil phenyl HPLC column (2.1 x 150 mm) from Thermo scientific. Samples were injected with a syringe loading injector fitted with a 100 μl loop.
Shimadzu Libror AEG-220 analytical balance (Shimadzu Corporation, Japan) was used to prepare standard solutions. Purified deionized water was obtained from Labconco Water Pro-PS UV ultra filtered water system (Labconco Corporation, Missouri) and distilled water was obtained by Aquatron A4S water system. Micro-centrifugation was performed using a BioFuge-Pico D-37520 centrifuge (Heraeus Instruments, Germany).