Igg2b mab

Manufactured by Abcam

IgG2b mAb is a class of monoclonal antibodies with an IgG2b isotype. Monoclonal antibodies are laboratory-produced molecules that can bind to specific targets within the body.

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Lab products found in correlation

Nb600 308, by Novus Biologicals (1 mentions) Odyssey infrared imager, by LI COR (1 mentions) Sds page gel, by Bio-Rad (1 mentions) Laemmli sample buffer, by Bio-Rad (1 mentions) Ma1 91878, by Thermo Fisher Scientific (1 mentions) Immobilon p pvdf membrane, by Merck Group (1 mentions) Ab18255, by Abcam (1 mentions)

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IgG2b (13 citations)

2 protocols using igg2b mab

Whole Worm Lysate Preparation and Analysis

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Whole worm lysates were generated from indicated worms. ~100 worms were collected, washed in M9 buffer and resuspended in equal volume of 2X Laemmli sample buffer (Bio-RAD). Worm lysates or E3 ligase reaction mixtures were resolved on 4–20% stain-free SDS-PAGE gels (Bio-RAD) and transferred to Millipore Immobilon-P PVDF membranes. Membranes were blocked with 5% nonfat milk and probed with mouse anti-FLAG (MA1-91878; Invitrogen; 1:1000; RRID AB_1957945), rabbit anti-GFP (NB600-308; Novus Biologicals; 1:2000; RRID: AB_10003058), mouse anti-HA [12CA5; amino acids 98–106 of human influenza virus hemagglutinin protein; IgG2b mAb; 1:1000; RRID: AB_2532070; in-house (Trimmer Laboratory)], or anti-Histone-H2A (ab18255; Abcam; 1:1000; RRID:AB_470265) followed by IRDye800-conjugated anti-mouse IgG secondary antibodies (962 32212; LI-COR Bioscience; 1:20000; RRID: AB_621847). Immunoblots were imaged on a LI-COR Odyssey Infrared Imager, signal was quantified using Fiji and normalized with total protein stain.

Li Q., Kaur A., Okada K., McKenney R.J, & Engebrecht J. (2023). Differential requirement for BRCA1-BARD1 E3 ubiquitin ligase activity in DNA damage repair and meiosis in the Caenorhabditis elegans germ line. PLOS Genetics, 19(1), e1010457.

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Anti-ganglioside Antibody Inhibition of Axon Regeneration

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Two disease relevant anti-ganglioside monoclonal antibody (mAb), GD1a/GT1b-2b (a prototypic IgG2b mAb against GD1a/GT1b) and GT1b-2b (IgG2b mAb with specificity for GT1b), were used in this study. We have demonstrated that these mAbs inhibit axon regeneration, prevent target re-innervation and induce injury to intact nerve fibers in different animal models (He et al., 2015 (link);Lehmann et al., 2007 (link);Zhang et al., 2011 (link)). The generation, specificity, and production, of these mAb were reported previously (Lunn et al., 2000 (link);Schnaar et al., 2002 ). The hollow fiber supernatant containing anti-ganglioside mAb was used in all animal studies. An irrelevant mouse isotype matched IgG-2b mAb (Abcam, Cambridge, MA) was used as a negative control.

Zhang G., Massaad C.A., Gao T., Pillai L., Bogdanova N., Ghauri S, & Sheikh K.A. (2016). Sialylated intravenous immunoglobulin suppress anti-ganglioside antibody mediated nerve injury. Experimental neurology, 282, 49-55.

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