Cells were grown on glass coverslips, fixed in 4% formaldehyde for 10 min, permeabilized with 0.1% Triton X-100 for 5 min, blocked in 10% normal goat serum, and incubated with primary antibodies (1:200) overnight at 4 °C. The samples were then treated with rhodamine/FITC-conjugated secondary antibodies (1:400, Sigma-Aldrich, St. Louis, MO, USA) and mounted with Vectashield mounting medium containing 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich). Images were captured with a confocal microscope (AX10, Carl Zeiss, Gottingen, Germany).
Rhodamine fitc conjugated secondary antibodies
Manufactured by Merck Group
Sourced in United States
Rhodamine/FITC-conjugated secondary antibodies are fluorescently labeled antibodies used in various immunoassays and imaging techniques. They bind to the primary antibody and emit a specific fluorescent signal, allowing the detection and visualization of target molecules.
Automatically generated - may contain errors
2 protocols using rhodamine fitc conjugated secondary antibodies
1
Immunofluorescence Staining of Cells
2
Immunophenotyping of PDLSCs
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PDLSCs were cultured on 24-well plates with glass coverslips at a density of 105 cells/well overnight. Then, PDLSCs were fixed with 4% paraformaldehyde and incubated with anti-CD146 (1:100; Abcam, USA), anti-CD105 (1:100; Abcam, USA), and anti-CD45 (1:200; Abcam, USA) primary antibodies. The samples were then treated with rhodamine/FITC-conjugated secondary antibodies (1:1000; Sigma-Aldrich, USA) and stained with 4,6-diamidino-2-phenylindole (Sigma-Aldrich, USA). Jurkat cells were stained with anti-CD45 antibody as a positive control. The images were captured with a fluorescence microscopy (OLYMPUS, Japan).
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