Holo transferrin bovine

The phospholipids, dioleoyl-3-trimethylammonium-propane chloride (DOTAP), dioleoyl-snglycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) were purchased from Avanti Polar Lipids (Birmingham, AL, USA). The phospholipid DSPE–PEG₂₀₀₀–NHS was purchased from Biochempeg Scientific Inc (Watertown, MA, USA). Holo-transferrin bovine, cholesterol, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), Ethylenediaminetetraacetic acid (EDTA), 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI), Hoechst 33342, Ethidium bromide (EtBr), Sodium azide, Amiloride and Triton™ X-100 were obtained from Sigma–Aldrich (St. Louis, MO, USA). Chlorpromazine and Colchicine were obtained from Enzo Life Sciences (Farmingdale, NY, USA). Chitosan (MW 30 kDa) was purchased from Glentham Life Sciences (Corsham, UK). Plasmid DNA encoding β-galactosidase (gWiz-βGal) and plasmid DNA encoding Green Fluorescent Protein (gWiz-GFP) were purchased from Aldevron LLC (Fargo, ND, USA). Dulbecco’s Modified Eagle Medium (DMEM), and phosphate buffered saline (PBS) were purchased from Corning Incorporated (Corning, NY, USA). Fetal bovine serum (FBS) was purchased from JR Scientific Inc. (Woodland, CA, USA). β-galactosidase enzyme assay kit with reporter lysis buffer was supplied by Promega (Madison, WI, USA).