19 (link),20 (link)Chemicals were obtained from Sigma-Aldrich (St. Louis, MO) unless otherwise noted. Flash chromatography was conducted using silica gel (200–300 mesh). Thin-layer chromatography (TLC) was visualized by UV and/or staining by 2,6-dibromoquinone-4-chloroimide (DBQ) or iodine. The 1H-,19 (link)F-, and31 P NMR spectra were recorded on a Varian Avance 400-MHz spectrometer. High resolution mass spectrometry was performed with a Micromass LCT - Waters 2795 HPLC with a 2487 UV detector using caffeine as a molecular weight standard. Biochemical reagents were purchased from Fisher Scientific or Sigma-Aldrich (St. Louis, MO). Electric eel acetylcholinesterase (EEAChE), human recombinant acetylcholinesterase (HuAChE), human serum butyrylcholinesterase (HuBChE), acetylthiocholine iodide (ATChI), S-butyrylthiocholine iodide (BTChI), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), and asolectin were purchased from Sigma-Aldrich.
Avance 400 mhz spectrometer
Manufactured by Agilent Technologies
Sourced in Japan, Germany
The Avance 400-MHz spectrometer is a nuclear magnetic resonance (NMR) instrument designed for analytical applications. It operates at a frequency of 400 MHz and is capable of performing high-resolution NMR measurements on a variety of sample types.
Automatically generated - may contain errors
Lab products found in correlation
Asolectin, by Merck Group (1 mentions)
Micromass lct waters 2795 hplc, by Waters Corporation (1 mentions)
Rf 5301pc, by Shimadzu (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Cyclohexanone, by Merck Group (1 mentions)
Digital ph meter, by Merck Group (1 mentions)
6540 uhd q tof lc ms, by Agilent Technologies (1 mentions)
F 4600 spectrofluorometer, by Hitachi (1 mentions)
2400 series 2 chns o analyzer, by PerkinElmer (1 mentions)
6224 tof lc ms instrument, by Agilent Technologies (1 mentions)
4 protocols using avance 400 mhz spectrometer
1
Synthesis and Characterization of Cholinesterase Inhibitors
2
Synthesis and Characterization of Fluorescent Probes
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Cyclohexanone
(Sigma Aldrich), 4-dimethyl aminobenzaldehyde (Alfa Aesar), NaOH (Merck),
and all metal salts (BDH chemicals England) in the form of chloride
or nitrate including Cd(NO3)2·4H2O, Hg(NO3)2, Co(NO3)2·4H2O, Ce(NO3)3·6H2O, Zn(NO3)2·6H2O, CuCl2·2H2O, Mn(NO3)2·4H2O, NiCl2·6H2O, Cr(NO3)3, and Pb(NO3)3 were used. All
the chemicals were used without any further purification. Solution
pH was measured with a digital pH meter (Merck). For fluorescence
and absorption spectral measurements, a spectrophotofluorometer RF-5301
PC (Shimadzu, Japan) and 1601 Double-beam UV–visible Spectrophotometer
(Tokyo, Japan) was used. 1H-NMR and FTIR spectra were taken
on a Bruker Avance 400 MHz spectrometer (Varian) and FTIR instrument
Prestige 21 (Shimadzu, Japan) in the 400–4000 cm–1 range, respectively. All chemical shifts were recorded on the δ-scale
in deuterated chloroform (CDCl3) solvent.
(Sigma Aldrich), 4-dimethyl aminobenzaldehyde (Alfa Aesar), NaOH (Merck),
and all metal salts (BDH chemicals England) in the form of chloride
or nitrate including Cd(NO3)2·4H2O, Hg(NO3)2, Co(NO3)2·4H2O, Ce(NO3)3·6H2O, Zn(NO3)2·6H2O, CuCl2·2H2O, Mn(NO3)2·4H2O, NiCl2·6H2O, Cr(NO3)3, and Pb(NO3)3 were used. All
the chemicals were used without any further purification. Solution
pH was measured with a digital pH meter (Merck). For fluorescence
and absorption spectral measurements, a spectrophotofluorometer RF-5301
PC (Shimadzu, Japan) and 1601 Double-beam UV–visible Spectrophotometer
(Tokyo, Japan) was used. 1H-NMR and FTIR spectra were taken
on a Bruker Avance 400 MHz spectrometer (Varian) and FTIR instrument
Prestige 21 (Shimadzu, Japan) in the 400–4000 cm–1 range, respectively. All chemical shifts were recorded on the δ-scale
in deuterated chloroform (CDCl3) solvent.
3
Synthesis and Characterization of 3-Cyanoacetylindole Derivatives
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All commercially available chemicals were purchased from Nanjing Crystal Chemical Co., Ltd. (Nanjing, China) or Alfa Aesar (Beijing, China) and were analytically pure. The specification of silica gel for column chromatography was 200–300 mesh. All target compounds were characterized by melting point, 1H NMR, 13C NMR, and HR-MS (ESI). The instruments were Büchi M-560 melting point apparatus, Bruker Avance 400 MHz spectrometer (Rheinstetten, Germany), Agilent Technologies 6540 UHD Q-TOF LC-MS (Palo Alto, CA, USA).
Furthermore, 3-cyanoacetylindole (2 ) and the target compounds (3 ) were synthesized using the reported methods [32 (link),33 (link)]. All of the reaction yields were not optimized.
Furthermore, 3-cyanoacetylindole (
4
Multimodal Characterization of Organic Compounds
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1H NMR spectra were obtained by the Bruker Avance 400 MHz spectrometer in d6-DMSO, and high-resolution mass (HRMS) spectra were obtained using the Agilent 6224 TOF LC/MS instrument. Fourier transform infrared (FTIR) spectroscopy was conducted using the NEXUS 870 FTIR spectrophotometer, and fluorescence spectra were obtained using the Hitachi F-4600 spectrofluorometer. Quantum yields were determined with respect to quinine sulfate (ΦF = 0.546) using the standard method;34–36 (link) the X-ray crystallography data and the results of elemental analyses were obtained using the Bruker Apex II X-ray diffractometer and the PerkinElmer Series II CHNS/O 2400 analyzer, respectively. The images of living cells were obtained using the Cyration 5 confocal microscope.
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1H NMR spectra were obtained by the Bruker Avance 400 MHz spectrometer in d6-DMSO, and high-resolution mass (HRMS) spectra were obtained using the Agilent 6224 TOF LC/MS instrument. Fourier transform infrared (FTIR) spectroscopy was conducted using the NEXUS 870 FTIR spectrophotometer, and fluorescence spectra were obtained using the Hitachi F-4600 spectrofluorometer. Quantum yields were determined with respect to quinine sulfate (ΦF = 0.546) using the standard method;34–36 (link) the X-ray crystallography data and the results of elemental analyses were obtained using the Bruker Apex II X-ray diffractometer and the PerkinElmer Series II CHNS/O 2400 analyzer, respectively. The images of living cells were obtained using the Cyration 5 confocal microscope.
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