Tin protoporphyrin 9 snpp

Fetal bovine serum (FBS) was purchased from Hyclone (Thermo, Melbourne, Australia). RPMI1640 medium (no phenol red), penicillin and streptomycin were purchased from Invitrogen (Grand Island, NY, USA). Recombinant GM-CSF and IL-4 were from Peprotech (Rocky Hill, NJ). Lipopolysaccharide (LPS) derived from Escherichia coli 026:B6 was from Sigma (St. Louis, MO). FITC-CD80, PE-CD86, FITC-MHCII or respective isotypes were from eBioscience (San Diego, CA, USA). Rabbit anti-mouse heme oxygenase 1(HO-1), rabbit anti-mouse β-actin and goat anti-rabbit IgG-horseradish peroxidase (HRP) were from Bioworld (St. Louis Park, MN, USA). Micro BCA protein assay kit was from Pierce (Rockford, IL). Cobalt protoporphyrin (CoPP), an inducer of HO-1 [38 (link)], and tin protoporphyrin IX (SnPP), an inhibitor of HO-1 activity [38 (link)], were from Sigma. Synthetic BP5 was purchased from Biotech Bioscience and Technology Co., Ltd (Shanghai, China). The purity of the synthetic peptide was > 98% by RP-HPLC. The sequence of the synthetic peptide was examined by electrospray ionisation tandem mass spectrometry (ESI-MS/MS). It was also tested the LPS contamination using the E-Toxate Limulus LPS detection kit (Sigma), which is sensitive to 0.05 to 0.1 endotoxin units/ml. Only uncontaminated preparations were used.

Paeoniflorin (purity: 98.04%) was acquired from MedChemExpress (Monmouth Junction, NJ, United States). Tin protoporphyrin IX (SnPP) and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (St. Louis, MO, United States). Primary antibodies were used as following: C-caspase3 (ab32351), Bax (ab32503), Bcl-2 (ab182858), and Cytochrome C (ab133504) were obtained from Abcam (Cambridge, United Kingdom); Keap1 (sc-514914) and poly (ADP-ribose) polymerase (PARP) (sc-7150) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, United States). Primary antibodies against Nrf2 (16396-1-AP), HO-1 (27282-1-AP), Lamin B (12987-1-AP), NF-κB (66535-1-Ig) and β-actin (66009-1-Ig) were acquired from Proteintech Group (Chicago, IL, United States), and 4′, 6-diamidino-2-phenylindole (DAPI) was received from Beyotime (Shanghai, China). ML385 was purchased from ChemeGen (Shanghai, China). All experimental consumables for cell culture are from Gibco (Grand Island, NY, United States).